Elicitation effects of methyl jasmonate on iridoid biosynthesis in leaves of Nepeta rtanjensis and Nepeta nervosa
2022
Аутори:
Aničić, NedaFilipović, Biljana
Dmitrović, Slavica
Nestorović Živković, Jasmina
Skorić, Marijana
Gašić, Uroš
Milutinović, Milica
Matekalo, Dragana
Petrović, Luka
Božunović, Jelena
Banjanac, Tijana
Šiler, Branislav
Todorović, Miloš
Lukić, Tamara
Mišić, Danijela
Остала ауторства
Milutinović, MilicaТип документа:
Конференцијски прилог (Објављена верзија)
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© 2022 by the Serbian Plant Physiology Society
Метаподаци
Приказ свих података о документуАпстракт:
The genus Nepeta L. comprises medicinal plant species that synthetize terpenes and phenolics of therapeutic value. Considerable variability in qualitative and quantitative content of iridoid
monoterpenoids has been detected within the genus Nepeta, which comprises iridoid produc-ing
and non-producing species. Nepeta rtanjensis Diklić & Milojević is an endemic plant of the Balkan Peninsula, which produces both iridoid aglycones (IAs) and iridoid glucosides (IGs). N. nervosa
Royle ex Benth., endemic to the Himalayas of Kashmir, India, and Paki-stan, is iridoid-lacking species. This study aimed to comparatively analyze the effect of me-thyl jasmonate (MeJA), an elicitor of terpene production in many plant species, on iridoid bio-synthesis at the molecular level.
Iridoid content in MeJA-treated N. rtanjensis and N. nervosa plants under in vitro conditions was
analysed in parallel with the co-expression patterns of iridoid related biosynthetic genes (GPPS,
GES, G80, 8HGO, 7DLGT, IS, and NEPS), major latex protein-like gene (MLPL), and genes coding for
different transcription factors (MYC2, YABBY5, COI1, and JAZ3). In general, the exogenous application of 250 µM MeJA for 2 h, 24 h and 72 h resulted in an increased accumulation of targeted IAs
(trans, cis-nepetalactone and 5,9-dehydronepetalactone) and IGs (1,5,9-epideoxyloganic acid) in
leaves of N. rtanjensis, as a result of elevated expression of the key biosynthetic genes. Interestingly,
although the majority of iridoid-related biosynthetic genes and TFs were identified in transcriptome of N. nervosa leaves, and the expression of some of them (NEPS and JAZ3) is up-regulated
follow-ing MeJA treatment, no iridoids were accumulated in leaves of N. nervosa.
Кључне речи:
Nepeta rtanjensis; Nepeta nervosa; chemotypes; methyl jasmonate; iridoid biosynthesisФинансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
- NEPETOME - Omics-guided disentangling of the iridoid diversity in the genus Nepeta L. for in planta metabolic engineering towards sustainable exploitation (RS-ScienceFundRS-Ideje-7749433)
У:
- Milutinović M, editor. Book of Abstracts: 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. Belgrade: Serbian Plant Physiology Society; 2022. p. 122.