Expression profiles of the bioactive gibberellin metabolism key genes in the spinach root-tips during somatic embryo induction
2019
Authors:
Belić, MajaZdravković-Korać, Snežana
Milojević, Jelena
Contributors
Hedden, PeterStrnad, Miroslav
Thomas, Stephen
Document Type:
Conference object (Published version)
,
© 2019 by the Faculty of Science, Palacký University Olomouc; Institute of Experimental Botany, Czech Academy of Sciences
Metadata
Show full item recordAbstract:
Gibberellins (GA) play a pivotal role in the induction of somatic embryogenesis (SE) from the apical root sections of spinach seedlings. To study the role of GA in this process, expression of genes encoding the key enzymes that catalyze the final step of bioactive GA biosynthesis (GA20-ox and GA3-ox) and degradation (GA2-ox1, -ox2 and -ox3) was analysed in the explants cultivated on inductive medium (IM - supplemented with 20 μM NAA + 5 μM GA3), noninductive medium (NM - containing 20 μM NAA), or plant growth regulator (PGR)-free medium. Expression of these genes was monitored by means of quantitative real-time PCR during 28 days SE-induction period. Root-tips isolated from seedlings and immediately frozen for RNA isolation were used as a control. In the IM-cultivated explants, expression of both GA20-ox and GA3-ox decreased over the whole induction period. On the other hand, in the explants cultivated on NM or PGR-free medium expression of these genes began to increase after 1 or 14 days, respectively, and remained higher than in the control steadily over time. Concerning the catabolic GA2-ox genes, particularly GA2-ox2, their expression was higher in the IM-cultivated explants over the whole SE-induction period comparing to the control. However, in the NM-cultivated explants, expression of GA2-ox2 and GA2-ox3 was mostly lower. The results indicate that continuous expression level decrease in GA20-ox and GA3-ox and increase in GA2-ox2 favor SE regeneration from root-tips of spinach. Understanding the role of GA in SE induction in spinach would not only provide a deeper insight into the mechanism of this process, but also enhance the improvement of this species through biotechnological approach.
Keywords:
spinach; somatic embryogenesis; gibberellins; gene expressionFunding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
In:
- Hedden P, Strnad M, Thomas S, editors. Book of Abstracts: Gibberellins 2019 – Current Progress in Gibberellin Research; 2019 Jun 30 – Jul 2; Olomouc, Czech Republic. Olomouc: Faculty of Science, Palacký University Olomouc Institute of Experimental Botany, Czech Academy of Sciences; 2019. p. 39.