Pleiotropic effects of over-expressed heterologous proteinase inhibitors in transformed Lotus corniculatus L. plants
2014
Preuzimanje 🢃
Autori:
Ninković, SlavicaNikolić, Radomirka
Banjac, Nevena
Cingel, Aleksandar
Savić, Jelena
Ninković, Sofija
Raspor, Martin
Ćosić, Tatjana
Radović, Svetlana
Smigocki, Ann C.
Tip dokumenta:
Konferencijski prilog (Objavljena verzija)
,
© 2014 by the International Association for Plant Biotechnology
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Proteinase inhibitors (Pls) are small proteins that occur naturally in a number of plant species that are
characterized by varied specificity toward proteinases, among them insect digestive proteinases of the
serine, cysteine, aspartyl and meialloproteinase class. Over-expression of heterologous proteinase
inhibitor genes in transgenic plants was used as a strategy for introducing insect and nematode
control. Besides their main effects (biodefense), Pls are also known to have positive side-effects on
transgenic plant physiology. ln this study engineered bird's foot trefoil (Lofus corniculatus L.) plants
with three different PI genes - Beta vulgaris serine Pl, Nicotiana alata serine Pl and squash aspartyl Pl
- exhibited enhanced growth with higher number of tillers and increased fresh and dry weight.
Moreover, some of the transformed lines displayed higher tolerance to NaCl stress evaluated on the
basis of growth and associated parameters, such as salinity injury and chlorophyll content, using two
NaCl levels (100 and 200 mM) for up to 10 d. Overall, the results indicate that the over-expression of
heterologous proteinase inhibitors could be a promising approach for increasing productivity of
genetically improved Lotus corniculatus L. plants.
Finansiranje / projekti:
- Biotehnologija in vitro - gajene, lekovite i ugrožene biljne vrste (RS-MESTD-Basic Research (BR or ON)-173015)
U:
- Book of abstracts: International Association for Plant Biotechnology Congress 2014; 2014 Aug 10-15; Melbourne, Australia. International Association for Plant Biotechnology; 2014. p. 246.