Antioxidant properties of haptoglobin in experimental diabetes
2007
Аутори:
Grigorov, IlijanaArambašić Jovanović, Jelena
Bogojević, Desanka
Ivanović-Matić, Svetlana
Grdović, Nevena
Vidaković, Melita
Dinić, Svetlana
Uskoković, Aleksandra
Martinović, Vesna
Petrović, Miodrag
Poznanović, Goran
Mihailović, Mirjana
Тип документа:
Конференцијски прилог (Објављена верзија)
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© SAGE Publications
Метаподаци
Приказ свих података о документуАпстракт:
Oxidative stress was proposed as a key factor in the development of complications in diabetes. Antioxidant defense mechanisms pri- marly act through a broad spectrum of antioxidative enzymes, how- ever, other molecules could also express antioxidative properties. One such molecule is haptoglobin (Hp), a serum protein that func- tions as an antioxidant by virtue of its ability to bind hemoglobin and thereby prevent oxidative tissue damage. Elevation of serum Hp con- centration was obtained 12 h after acute tissue injury, and its level decreased after 24h to the control level. In our studies of streptozo- tocin-induced diabetes in adult male Wistar rats (i.p., 65mg/kg body weight), we found chronic but different degrees of increased serum Hp concentrations depending on time after treatment. The highest elevation (eight-fold), was obtained two weeks after treatment by rocket immunoelectrophoresys where the maximal activation of total SOD and superoxide anion was detected (ELISA) in the blood and in the liver, the central organ of Hp synthesis. At this time, most of the diabetic rats died suggesting that the early stage of diabetes was critical for survival. Four end seven weeks after treatment, expres- sion of Hp was still elevated, but less than after two weeks, as was the concentration of the superoxide anion and SOD activity. The strong correlation between the diabetes-related oxidative status and expression of Hp suggests that its antioxidative property plays an important role in the early stage of diabetes.
Финансирање / пројекти:
- Акутни и хронични стрес: механизми регулације хомеостазе у акутној радијационој болести и дијабетесу (RS-MESTD-MPN2006-2010-143002)
У:
- Abstracts of the 2nd International Congress on Prediabetes and the Metabolic Syndrome; 2007 Apr 25-28; Barcelona, Spain. SAGE Publications; 2007. S176. (Diabetes and Vascular Disease Research; March; Suppl. 1).