Cryonecrosis induces activation of nuclear matrix-associated endonuclease p23
2007
Аутори:
Dinić, SvetlanaGrdović, Nevena
Mihailović, Mirjana
Vidaković, Melita
Uskoković, Aleksandra
Martinović, Vesna
Arambašić Jovanović, Jelena
Grigorov, Ilijana
Bogojević, Desanka
Poznanović, Goran
Тип документа:
Конференцијски прилог (Објављена верзија)
,
© Federation of European Biochemical Societies
Метаподаци
Приказ свих података о документуАпстракт:
Repeated exposure of tissues to cryogenic temperatures induces
fast and massive necrosis. Previously we characterized p23 endonuclease
as Mg2+-dependent, sequence non-specific enzyme, associated
with the nuclear matrix isolated from cryopreserved liver.
Since p23 was absent in preparations from fresh liver, we supposed
that freezing/thawing induce its activation. Our aim was to establish
whether cryopreservation of liver results in necrotic changes
and is the activation of p23 related to this process. The nuclear
matrix was prepared from fresh and from livers subjected to one
and three freezing/thawing cycles. Necrosis was induced in vivo by
repeated freezing/thawing of liver tissue in an anesthetized rat. We
showed that both ex vivo and in vivo freezing/thawing of the liver
were accompanied by biochemical events commonly observed during
necrosis (increased LDH activity, DNA degradation, absence
of lamin proteolysis and typical PARP-1 necrotic cleavage). Using
the activity gel analysis, it was established that ex vivo and in vivo
freezing/thawing promoted the appearance of p23 activity,
although to lower extent in in situ injured liver. Our results
revealed that induction of cryonecrotic process in liver goes along
with activation of endonuclease p23.
У:
- 32nd FEBS Congress, Molecular Machines, July 7-12, Vienna, Austria. FEBSPress; 2007. p. 372. (Febs Journal; Vol 274; No. S1).