Pretreatment of diabetic rats with ethyl piruvate upregulates HMGB-driven protective autophagy in the liver
2018
Autori:
Petrović, AnjaBogojević, Desanka
Ivanović-Matić, Svetlana
Martinović, Vesna
Korać, Aleksandra
Jovanović-Stojanov, Sofija
Grigorov, Ilijana
Ostala autorstva
Đurić, Dragan M.Jakovljević, Vladimir
Živković, Vladimir
Tip dokumenta:
Konferencijski prilog (Objavljena verzija)
,
© 2018 by the Serbian Physiological Society
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Oxidative stress. cell damage and cell death underlies the etiology of liver damage/dysfunction in diabetes. Bigh Mobility Group Box 1 (HMGB1) is a nuclear protein that is released from damaged/stressed liver cells during, diabetes and contributes to oxidative stresse-mediated autophagy an cell death/survival. Since ethyl pyruvate (EP) an HMGB1 relase/expession and autophagy in the liver of diabetic rats. Diabetes was indused by streptozotocin(65mg/kg). Pretreated group of diabetes rats- (EP+D) startedl treatment with EP (80mg/kg/daily) three·days before
diabetes induction, while in the second group (D+EP} treatment start ten days after diabetes induuction. In compcarison with D+EP group, EP+D group had better glycemic status and higher activity of antioxidative enzyme SOD and CAT in diabetic liver. According to western immunoblot analyses, EP+D group sliowed higher expression of extracellular HMGB l in comparison with D+EP. Consequently detected increase in HMGB1/RAGE interactions in EP+D group were followed by higher expression of LC3-II, HMGB1/Beclin 1 interaction and activation of autophagy. Expression of LC3-II and HMGB1/Beclin 1 interaction were at the control level in D+EP. Preserved liver morphology in both EP treated groups, observed by electron microscopy, implicated existence of adaptive mechanisms in EP+D group. Further analyses showed that protective autophagy (mitophagy) was enhanced in EP+D group compared with diabetic and D+EP groups. Thus treatment of diabetic patients with EP may constitute a new strategy for the treatment of diabetes related tissue injury.
Finansiranje / projekti:
- Signalni molekuli u dijabetesu: identifikacija potencijalnih bioloških markera uključenih u modifikaciju i integraciju signalnih puteva u cilju predikcije i intervencije u dijabetesu (RS-MESTD-Basic Research (BR or ON)-173020)
U:
- Đurić DM, Jakovljević V, Živković V, editors. Abstract book: 4th Congress of Physiological Sciences of Serbia with International Participation: Current Trends in Physiological Sciences: from cell signals to the biology of aging; 2018 Sep 19-23; Niš, Serbia. Belgrade: Serbian Physiological Society; 2018. p. 108.