Micropropagation of Clinopodium thymifolium (Scop.) Kuntze (Lamiaceae)
2022
Autori:
Stojičić, DraganaTošić, Svetlana
Zlatković, Bojan
Blagojević, Saša
Manić, Branislav
Budimir, Snežana
Uzelac, Branka
Ostala autorstva
Ranđelović, VladimirStojanović-Radić, Zorica
Nikolić, Danijela
Jenačković Gocić, Dragana
Tip dokumenta:
Konferencijski prilog (Objavljena verzija)
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© 2022 by the Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Clinopodium thymifolium (Scop.) Kuntze (syn. Micromeria thymifolia (Scop.) Fritsch) is a Balkan endemic species, traditionally used as a condiment and medicinal plant in the Mediterranean area. Clinopodium species were shown to produce considerable quantities (> 0.5%) of essential oils. The essential oil of wild-growing C. thymifolium possesses high antimicrobial activity. One alternative for the production of commercially important plant-derived metabolites, especially of rare plant genotypes, is in vitro plant tissue culture, given that the metabolic potential of in vitro shoot cultures can be manipulated by varying in vitro culture conditions. In view of the potential pharmacological and commercial value of C. thymifolium, the present study was initiated to propagate this aromatic plant species with the aim to increase the accumulation of biomass and the production of secondary metabolites. Shoots of wild-growing plants, dissected into one-node stem segments bearing two axillary buds, were used to establish in vitro cultures. Shoot multiplication was carried out on basal nutritional medium and on medium supplemented with various plant growth regulators at different concentrations. Nodal segments developed axillary shoots, and were successfully propagated on basal medium, whereas tested plant growth regulators differently affected their multiplication and biomass production.
Ključne reči:
axillary bud induction; in vitro propagation; shoot multiplication; biomass production; cytokininsFinansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200007 (Univerzitet u Beogradu, Institut za biološka istraživanja 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200124 (Univerzitet u Nišu, Prirodno-matematički fakultet) (RS-MESTD-inst-2020-200124)
U:
- Ranđelović V, Stojanović-Radić Z, Nikolić D, Jenačković Gocić D, editors. Book of abstracts: 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions; 2022 Jun 26-29; Kladovo, Serbia. Niš: Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš; 2022. p. 113.