Supplementary material for the article:Beyond Antioxidant Activity: Redox Properties of Catechins May Affect Changes in the DNA Methylation Profile—The Example of SRXN1 Gene

Abstract

Table S1. Primer sequences for SRXN1 gene used in MSP and MS-HRM. Table S2. Primer sequences for SRXN1 and GAPDH genes used in RT-qPCR. Table S3. Methylation changes in CpG island within the promoter region of SRXN1 in HT29 cell line investigated with MSP. Cells were treated with catechins and glutathione at concentrations of 0.1, 1,10, and 100 µM for 24 h at 37°C. The results are means ± SD of three biological replicates. Table S4. Methylation changes in CpG island within the promoter region of SRXN1 in HT29 cell line investigated with MS-HRM. Cells were treated with catechins and glutathione at concentrations of 0.1,1, 10, and 100 µM for 24 h at 37°C. The results are means ± SD of three biological replicates. Table S5. Changes in SRXN1 gene expression analysed with the RT-qPCR method. Expression levels were calculated using relative delta delta Ct method. Data was normalized to GAPDH gene expression. The results are means ± SD of three biological replicates. Figure S1. Inhibition of growth of HT29 cells determined by MTT assay after 72 h treatment with 5-Aza. Viability is expressed as a percentage relative to control cells (100%). EC30 and EC45 are the effective concentrations of 30% and 45% of cell growth inhibition. Results are means of three independent experiments carried out in triplicate (SD < 15%). Figure S2. The increase in DNA methylation level of SRXN1 gene determined by MS-HRM using M1/U1 primer sets following 24 h treatment of HT29 cells with catechins that increase methylation profile of SRXN1 promoter. Figure S3. The increase in DNA methylation level of SRXN1 gene determined by MS-HRM using M3/U3 primer sets following 24 h treatment of HT29 cells with catechins that increase methylation profile of SRXN1 promoter.