The analysis of fecal microbiota and insulin production in diabetic rats after oral administration of probiotic Lactobacillus paraplantarum BGCG11
2019
Authors:
Živković, MilicaSoković Bajić, Svetlana
Tolinački, Maja
Brdarić, Emilija
Đokić, Jelena
Popović, Nikola
Rajić, Jovana
Đorđević, Marija
Golić, Nataša
Document Type:
Conference object (Published version)
,
© 2020 Wolters Kluwer Health, Inc.
Metadata
Show full item recordAbstract:
Objective
Our previous studies with Lactobacillus paraplantarum BGCG11 probiotictreatment
of diabetic rats showed decreased hyperglycemia and
ameliorating effect on diabetes-associated damage of liver and kidneys.
Hence, the aim of this study was to reveal the effects of BGCG11 probiotic
on gut microbiota composition and monitoring the insulin production in
pancreatic islets in diabetic rats.
Methods
Experiments were performed on albino Wistar rats divided into four groups:
ND – non-diabetic control, D – streptozotocin (STZ) induced diabetes;
P/D/P – BGCG11 pretreatment; D/P – BGCG11 treatment. The rats were
orally administered with BGCG11, one week before (P/D/P) and after the
STZ injection, for four weeks (P/D/P and D/P). Total DNA was isolated from
all fecal samples and rDNA amplicons were analyzed by DGGE and 16S
rDNA genes sequencing. For immunohistochemical analysis, slides were
stained with anti-insulin antibody and secondary antibody coupled with
horseradish peroxidase.
Results
The results revealed the higher diversity of gut microbiota in D/P group
comparing to D group, as well as the higher prevalence of Flintibacter
butyricus (the major butyric producer), Acetatifactor muris (present in
obese mouse) and Eisenbergiella massiliensis (found in obese woman),
while the lipolytic bacterium Aestuariispira insulae was more prevalent in
diabetic rats. In both, P/D/P and D/P group, increased number of positive
immunoreactions of β-cells for anti-insulin antibodies was displayed in
compare to D group with islet atrophy.
Conclusions
The results of this study suggest that the positive effect of BGCG11 on
STZ-induced diabetes in rats could be annotated to its protective role on
the integrity of fecal microbiota.
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
In:
- 10th Probiotics, prebiotics and new foods, nutraceuticals and botanicals for nutrition and human and microbiota health and 1st Science; 2019 Sep 8-10; Rome, Italy. Wolters Kluwer Health; 2020. p. 103. (Journal of Clinical Gastroenterology; Vol. 54; Suppl. 1)