In Vitro Shoot Multiplication and Regeneration of the Recalcitrant Rocket (Eruca sativa Mill.) Variety Domaća Rukola
2023
Аутори:
Banjac, NevenaKrstić-Milošević, Dijana
Mijalković, Tatjana
Petrović, Mirjana
Ćosić, Tatjana
Stanišić, Mariana
Vinterhalter, Branka
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Eruca sativa is known in traditional medicine for its therapeutic effects, while young plants
are used as a salad or green food. Recently, the consumption of rocket has increased considerably,
so it has become very important for breeders. Plant tissue culture provides a platform to overcome
the problems in improving this species. In the present study, an efficient protocol for in vitro shoot
regeneration and propagation of recalcitrant rocket variety Domaća rukola was studied. Murashige
and Skoog (MS, 1962) medium containing 0.1 mg/L BA and frequent subculture over a period
of three weeks proved to be optimal for shoot multiplication with a multiplication index of over 3
and only 8.72% of hyperhydrated shoots without necrosis. Different concentrations of 2,4-D, BA, or
TDZ in combination with NAA, with or without the presence of AgNO3, were tested for de novo
shoot organogenesis (DNSO) from seedling explants. The hypocotyl explants cultured on MS with a
combination of TDZ1.0+NAA0.1+AgNO3 5.0 mg/L regenerated viable shoots with the highest rate
(25.38%) and an average number of 2.18 shoots per regenerating explant. Somatic embryogenesis
from immature zygotic embryos proved to be the best way to regenerate a recalcitrant rocket cultivar.
The highest embryogenic efficiency was achieved in explants cultured on MS medium containing
1.0 mg/L 2,4-D with a frequency of 76.64% and 5.13 mean number of regenerated somatic embryos
per explant, which were further converted into normal plants. Additionally, in vitro-produced rocket
shoots could serve as a possible promising source for the production of flavonoid kaempferol with
proven antioxidant properties.
Кључне речи:
organogenesis; somatic embryogenesis; cytokinins; rooting; kaempferolИзвор:
Horticulturae, 2023, 9, 5, 533-Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)