Selected terpenoids as obstructers of Candida virulence
2022
Аутори:
Ivanov, MarijaCarević, Tamara
Kostić, Marina
Ćirić, Ana
Petrović, Jovana
Glamočlija, Jasmina
Soković, Marina
Остала ауторства
Rajković, Miloš B.Popović-Đorđević, Jelena B.
Kostić, Aleksandar Ž.
Тип документа:
Конференцијски прилог (Објављена верзија)
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© 2022 by the University of Belgrade, Faculty of Agriculture
Метаподаци
Приказ свих података о документуАпстракт:
Terpenoids are the most complex class of natural products with over 80000 identified representatives found in plants, microorganisms, insects, and marine invertebrates. Their anticandidal potential, especially impact on fungal virulence is, however, not fully explored yet. We aimed to investigate anticandidal, antibiofilm, and antihyphal properties of selected terpenoids (borneol, thymol, geraniol, and carvacrol). The strongest antifungal potential towards Candida albicans strains could be noticed for borneol (lowest minimal inhibitory concentration, MIC, 0.06 mg/mL) followed by thymol (lowest MIC 0.12 mg/mL). On the other hand, MIC of geraniol was up to 8 mg/mL. Non-albicans Candida strains were most susceptible to thymol (MIC 0.12 mg/mL towards C. krusei H1/16) and borneol (MIC 0.12 mg/mL towards C. parapsilosis ATCC 22019). When the terpenoids were applied in their previously determined minimal inhibitory concentrations the strongest reducing impact on C. albicans ATCC 10231 biofilm forming ability was observed for carvacrol (60% inhibition), followed by geraniol (56% inhibition). Lower concentration of terpenoids (0.5 MIC) led to similar impairment of biofilm formation – the strongest impact was noticed for geraniol (54% inhibition), followed by carvacrol (47% inhibition) as determined by crystal violet assay. MTT antibiofilm assay highlighted great potential of carvacrol and geraniol to reduce viability of Candida cells in biofilms. Inhibition of hyphal formation could be noticed only upon application of carvacrol (14.29% of hyphal cells compared to the untreated control with 50.67% hyphae) suggesting wide antivirulence potential exhibited by this molecule. Other compounds explored in this study have displayed high MIC values (geraniol), low antibiofilm potential (thymol, borneol) or lack of antihyphal properties (all except carvacrol). Potential of carvacrol to reduce fungal virulence should be further studied on molecular level and in in vivo assays.
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
У:
- Rajković MB, Popović-Đorđević JB, Kostić AŽ, editors. Book of abstracts: 1st European Symposium on Phytochemicals in Medicine and Food, 1-EuSPMF; 2022 Sep 7-9; Belgrade, Serbia. Belgrade: University of Belgrade, Faculty of Agriculture; 2022.