Differences in carbonic anhydrase 9 and 12 expression in lung adenocarcinoma and lung squamous cell carcinoma
Разлике у експресији карбонске анхидразе 9 и 12 у аденокарциному плућа и карциному сквамозних ћелија плућа
2022
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Authors:
Nedeljković, MilicaStojišić, Jelena
Tanić, Nasta
Murganić, Blagoje
Tomić, Tijana
Ademović, Nejla
Tanić, Nikola
Contributors
Arsenijević, NebojšaDocument Type:
Conference object (Published version)
,
© 2022 by the Serbian Society for Immunology, Molecular Oncology and Regenerative Medicine
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Show full item recordAbstract:
Lung cancer is the most frequently diagnosed and lethal malignancy in the
world. Lung adenocarcinoma (LAC) and lung squamous cell carcinoma (LSCC)
are two subtypes of non-small cell lung cancer (NSCLC) which differ markedly in
key clinical and biological features. Despite this, they are usually treated similarly.
It is imperative to elucidate the mechanisms behind these differences in order to
implement better therapeutic modalities and biomarkers. Increased expression of
carbonic anhydrases Ca9 and Ca12 was observed in a broad array of tumors. Ca9
and Ca12 have a crucial role in the maintenance of the neutral intracellular pH and
the acidic extracellular microenvironment which stimulates the proliferation and
metastasis of tumor cells. Our aim was to detect possible difference in expression
level of Ca9 and Ca12 in LAC and LSCC, and to investigate whether the expression
of Ca9 and Ca12 was associated with the clinical course and outcome. We evaluated
the Ca9 and Ca12 expressions in 71 lung cancers, 35 (49%) LAC and 36 (51%)
LSCC. After RNA isolation and reverse transcription, relative RNA expression level
was evaluated using Real Time PCR and TaqMan technology. Ca9 and Ca12 expression
status was calculated according to the 2-ΔΔCT method. We used median value
of expression to designate low and high expression groups. High level of Ca9 and
Ca12 expression were detected in 49% (35/71) and 48% (34/71) of NSCLC samples,
respectively. Low levels of expression were identified in the rest of the specimens.
High expression of Ca12 was associated with LSCC subtype (p<0.0001). No associations
between Ca9 or Ca12 expression and clinicopathological parameters were
detected when assessed independently. However, patients with high expression of
both Ca9 and Ca12 lived significantly shorter compared to the Ca9/Ca12 low expression
group, (p=0.02). Our results suggest that Ca12 expression contributes to
the differences observed between LAC and LSCC tumors. The upregulation of Ca12 may promote the aggressive behavior of NSCLC. Ca9-high/Ca12-high expression
constitutes a ‘high risk’ profile in NSCLC.
Keywords:
lung adenocarcinoma; lung squamous cell carcinoma; Ca9; Ca12; RNA expressionFunding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
In:
- Arsenijević N, editor. Abstract Book: First Serbian molecular medicine congress; 2022 Jun 16-18; Foča, Bosnia and Herzegovina. Serbian Society for Immunology, Molecular Oncology and Regenerative Medicine; 2022. p. 78-81.