DNA profiling in cancer research- diagnostic and prognostic value
ДНК профилисање у истраживањима рака- дијагностички и прогностички значај
2022
Тип документа:
Конференцијски прилог (Објављена верзија)
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© 2022 by the Serbian Society for Immunology, Molecular Oncology and Regenerative Medicine
Метаподаци
Приказ свих података о документуАпстракт:
Cancer development is a multistage process that results from an accumulation of
mutations. Since spontaneous mutation rates in human cells are considerably lower
than the large number of mutations observed in cancer cells, cancer cells must be
a manifestation of mutator phenotype. The mutator phenotype, also referred to as
genomic instability, designates the increased mutation rate that occurs in neoplastic
cells. The induction of genomic instability phenotype is emerging to be a crucial
early event in carcinogenesis that enables an initiated cell to evolve into a cancer cell
by achieving a greater proliferative capacity and genetic plasticity, which can overcome
host immunological resistance, localized toxic environments and a suboptimal
supply of micronutrients. Three distinct forms of genomic instability have been
identified, microsatellite instability (MIN), chromosomal instability (CIN) and single
nucleotide instability (SNI). It is of great importance for the determination of
therapy and for therapy outcome, which form of instability is present in cancer
cells. Is there are a way to determine the form of instability and measure it in relatively
simple one step procedure and at low cost? Yes, AP-PCR is a PCR-based DNA
fingerprinting method for DNA profiling that utilizes arbitrarily chosen primers to
co-amplify multiple and independent sequences under low stringency conditions
during the first cycles. The unbiased nature of AP-PCR profiling allows for the
screening of anonymous regions of a genome without any prior knowledge of its
structure and provides information about two distinct types of DNA alterations.
These alterations represent accumulation of changes in DNA sequence (qualitative
changes – MIN phenotype) that manifest as mobility shifts in the banding pattern
while amplifications or deletions of existing chromosomal material (quantitative
changes – CIN phenotype) are evident as altered band intensities in the banding
pattern. We applied AP-PCR to measure genomic instability in samples of patients
with Non-Small Cell Lung Cancer, Anaplastic Astrocytomas, Glioblastoma Multiforme,
Head and Neck Squamous Cell Carcinomas and their premalignant lesions leukoplakias. Moreover, we identified some unique genetic alterations that has never
been associated with this types of cancer before.
Кључне речи:
DNA profiling; genomic instability; AP-PCRФинансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
У:
- Arsenijević N, editor. Abstract Book: First Serbian molecular medicine congress; 2022 Jun 16-18; Foča, Bosnia and Herzegovina. Serbian Society for Immunology, Molecular Oncology and Regenerative Medicine; 2022. p. 120-3.