Early-onset and late-onset calorie restriction differently modulate anxiety-like bahavior in aging female Wistar rats
2022
Authors:
Prvulović, MilicaTodorović, Smilja
Milanović, Desanka
Vukojević, Anđela
Simeunović, Valentina
Jović, Milena
Mladenović, Aleksandra
Document Type:
Conference object (Published version)
,
© 2022 by the Federation of European Neuroscience Societies
Metadata
Show full item recordAbstract:
Calorie restriction (CR) is known to prolong lifespan and healthspan, - life period free of age-related diseases. However, generality of CR’s beneficial effect is being questioned recently. Few studies showed that the outcome of CR could vary depending on its onset and duration. Herein, we examined and compared potential of different CR paradigms to modulate anxiety-like behavior. Methods: Ad libitum (AL) fed animals were used as controls. Female Wistar rats of different age (adult, middle-aged and aged) were exposed to CR (60% of AL), to examine the effect of early-onset CR (EOCR) and late-onset CR (LOCR). Open field (OF) and Light-dark box (LDB) test were used for anxiety assessment. Results: Aged
LOCR animals had decreased number of entries and time spent in the central area of the OF. LDB test results, however, implied a protective effect of EOCR since both middle-aged and aged EOCR animals showed increased number of entries in the light compartment and spent more time in the door area or in the light compartment. LOCR had different effect depending on animals’ age at the CR onset. Conclusions: EOCR ameliorates anxiety-like behavior and this effect seems to persevere till
old age. Implementing CR later in life should be taken with caution, since its’ effect could vary from protective to detrimental, depending on the onset. Additionally, different results were obtained using OF and LDB, suggesting that more than one test should be used to provide proper insight in certain behavioral changes.
Keywords:
Calorie restriction; Behaviour; AgeingFunding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
In:
- E-book of Abstracts: FENS Forum 2022; 2022 Jul 9-13; Paris, France. Federation of European Neuroscience Societies; 2022. S07-100.