Mechanism underlying effects of fish oil supplementation in presymptomatic stage of Alzheimer disease in 5XFAD mice
2019
Аутори:
Jović, MilenaLončarević-Vasiljković, Nataša
Ivković, Sanja
Milanović, Desanka
Kanazir, Selma
Остала ауторства
Isaković, AnđelkaТип документа:
Конференцијски прилог (Објављена верзија)
,
© 2019 by the Serbian Neuroscience Society
Метаподаци
Приказ свих података о документуАпстракт:
Aims: Alzheimer’s disease (AD) is the most prevalent type of dementia in elderly,triggered by multiple factors such as amyloid
plaques, neurofibrillary tangles and neuroinflammation. The use of supplements with omega-3 fatty acids has been associated
with reduced risk and lessened AD pathology. The purpose of this study was to elucidate the mechanisms underlying effects of
short-term fish oil (FO) suplementation in presymptomatic stage of AD in 5xFAD mice.
Methods: Three-month old female 5xFAD mice received FO (100μl/animal/day) via oral gavage during the period of 3 weeks.
Western blot and immunohistochemical analysis were used to detect changes in pathological features of AD in cortex of 5xFAD
mice. AmiloGlo was used to visualize plaques. Amyloid beta (Aβ) peptide, dystrophic neurites (DNs), microglia/macrophages
and CX3CR1/CX3CL1 were detect by anti-Aβ42-, anti-SMI31-, p-Tau-, anti-Iba-1-, anti-CX3CR1 anti-CX3CL1 antibodies,
retrospectively. Immunostaining was observed by confocal microscopy. Quantification was done by Image J and Image Quant
softwer.
Results: The present study shows that short-term FO supplementation applied in presymptomatic stage of AD, alters the
behaviour of microglia/macrophages prompting them to establish a physical barrier around amyloid plaques. This barrier
significantly suppresses DNs formation through the reduction of both Aβ content and tau hyperphosphorylation. Moreover, the
short-term FO treatment suppresses CX3CR1/CX3CL1 axis, interaction between microglial cells and neurons, which represents
one of possible mechanisms for altered microglial/macrophage motility and colocalization around plaques.
Conclusion: Our findings suggest that FO consumption may play an important role in modulating microglial response and
ameliorating the AD pathology in presymptomatic stage of Alzheimer’s disease.
Кључне речи:
Alzheimer's disease; 5xFAD mouse; Fish oilФинансирање / пројекти:
- Пластичност мозга током старења: утицај дијеталне рестрикције и анестезије (RS-MESTD-Basic Research (BR or ON)-173056)
У:
- Isaković A, editor. Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia. Belgrade: Serbian Neuroscience Society; 2019. p. 287.