Topographical effects of different anesthetics on GABAergic parvalbumin interneurons in rat
2023
Тип документа:
Конференцијски прилог (Објављена верзија)
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© 2023 by the Federation of European Neuroscience Societies
Метаподаци
Приказ свих података о документуАпстракт:
We examined the effects of ketamine/diazepam and propofol anesthesia on hippocampal and reticulo-thalamic (RT) GABAergic parvalbumin (PV)-expressing interneurons in the rat.
A total of 20 adult male Wistar rats were divided into two experimental groups - half were anesthetized with ketamine/diazepam (100 mg/kg, Zoletil® 50), and the other half received propofol anesthesia (100 mg/kg; Propofol Lipuro 2% (20mg/ml). We performed immunohistochemistry protocols for PV and postsynaptic density protein 95 (PSD-95) staining on free-floating 40-µm brain slices. We used PSD-95 as an excitatory synaptic marker to test local excitation changes along with changes in PV expression.
Our results show significant suppression of GABAergic PV-expressing interneurons during ketamine/diazepam anesthesia compared with propofol anesthesia, in the dentate gyrus and CA3 regions of the hippocampus, and in the RT. Moreover, this suppression resulted in an increase in PSD-95 expression only in the hippocampus of rats anesthetized with ketamine/diazepam. Topographically distinct effects of propofol anesthesia were not detected.
The observed imbalance between excitation and inhibition at the level of the hippocampus during ketamine/diazepam anesthesia could be a consequence of lower interneuronal GABA activity. Conversely, the topographically uniform expression of PSD-95 during propofol anesthesia together with higher expression of GABAergic interneurons could possibly indicate a stronger effect of GABA-mediated inhibition in the hippocampus compared with ketamine/diazepam anesthesia.
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
У:
- FENS Regional Meeting: FRM 2023; 2023 May 3-5; Algarve, Portugal. Federation of European Neuroscience Societies; 2023.