Primenjivost molekularnih metoda u identifikaciji vrsta slatkovodnih ekosistema
Using molecular methods in identification species of freshwater ecosystems
2014
Authors:
Raković, MajaPopović, Nataša
Đuknić, Jelena
Đikanović, Vesna
Atanacković, Ana
Paunović, Momir
Contributors
Đukić, AleksandarDocument Type:
Conference object (Published version)
,
© 2022 by the Serbian association for water protection
Metadata
Show full item recordAbstract:
The paper describes the use of mitochondrial markers cytochrome oxidase I of three freshwater limpet
(Ferrissia fragilis ( Tryon , 1863 ) , Ferrissia rivularis (Say 1817 ) and Ancylus fluviatilis (OF Müller ,
1774 )) for the assessment of the indigenous status of the investigated species in the Palearctic. The
analysis is based on COI gene sequences of 17 individuals from above mentioned species, taken from
the database "GenBank ". Analyses were performed on sequences of length 612 base pairs (bp). The
specimens used in the analysis originated from the area of North America as native population and they
were compared with alien Eurasian populations. Sample from species Ancylus fluviatilis was used as
outgroup taxon. Sequences were download in FASTA format and aligned using the ClustalW program
with predefined parameters. Phylogenetic analyses were conducted using MEGA 6. For making
phylogenetic trees we used two methods: "Neighbour - Joining“ and „Maximum Likelihood“.
Consensus trees from both applied methods showed that individuals of North American and Eurasian
populations had high degree of similarity, which confirms the hypothesis that analysed species originate
in North America and they are invasive in the Palearctic.
Keywords:
molecular methods; DNA barcoding; species identification; non-native species; genetic diversityFunding / projects:
- Geologic and ecotoxicologic research in identification of geopathogen zones of toxic elements in drinking water reservoirs- research into methods and procedures for reduction of biochemical anomalies (RS-MESTD-Basic Research (BR or ON)-176018)
- Biosensing Technologies and Global System for Long-Term Research and Integrated Management of Ecosystems (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-43002)
In:
- Đukić A, editor. Conference Proceedings 43rd Annual Conference of the Serbian Water Pollution Control Society: Water 2014; 2014 Jun 3-5; Belgrade, Serbia. Belgarde: Serbian Association for Water Protection; 2014. p. 141-8.