Chlamydia trachomatis infection triggers Epithelial Mesenchymal Transition in conjunctiva: involvement of DNA methylation
2017
Аутори:
Rajić, JovanaGrdović, Nevena
Inić-Kanada, Aleksandra
Stein, Elisabath
Schuerer, Nadine
Uskoković, Aleksandra
Ghasemian, Ehsan
Dinić, Svetlana
Mihailović, Mirjana
Arambašić Jovanović, Jelena
Tolić, Anja
Đorđević, Marija
Đorđević, Miloš
Poznanović, Goran
Barisani-Asenbauer, Talin
Vidaković, Melita
Остала ауторства
Mourdjeva, MilenaKistanova, Elena
Fazeli, Alireza
Brevini, Tiziana
Тип документа:
Конференцијски прилог (Објављена верзија)
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© 2017 COST Office: 2017
Метаподаци
Приказ свих података о документуАпстракт:
Repeated eye infections with Chlamydia trachomatis (Ct) usually lead to conjunctiva! scarring, corneal damage and blindness (trachoma). Considering epithelial to mesenchymal transition (EMD as unavoidable player in the development of fibrosis and taking into account that EMT is epigenetically regulated process, our intention was to explore whether Ct infection induces EMT in vitro and to uncover potential underlying epigenetic mechanisms.
Human conjunctiva! epithelial cells were infected with 107 IFU of Ct for 72 h. The mRNA and protein expression of EMT markers (E-cadherin and fibronectin) were assessed by RT-qPCR, lmmunoblotting and lmmunocytochemfstry. DNA methyfation analysis of selected regions of marker genes were examined by MSP, HRM and Bs-Seq.
The Ct infection induced EMT-related changes in mRNA and protein expression, downregulation of epithelial marker E-cadherin and upregulation of mesenchymal marker fibronectin. Increase in DNA methylation of E-cadherin gene promoter was in correlation with its decreased expression, while DNA methylation status of fibronectin gene couldn't be related to its expression level.
We suggest that manipulating the EMT process via modulation of DNA methylation of E-cadherin opens up possibilities to stop or revers the progression of scarring as a new strategy in trachoma treatment.
Финансирање / пројекти:
- European Cooperation in Science and Technology (COST) CA16119
- Сигнални молекули у дијабетесу: идентификација потенцијалних биолошких маркера укључених у модификацију и интеграцију сигналних путева у циљу предикције и интервенције у дијабетесу (RS-MESTD-Basic Research (BR or ON)-173020)
У:
- Mourdjeva M, Kistanova E, Fazeli A, Brevini T, editors. Proceedings of CellFit meeting: In vitro 3-D total cell guidance and fitness; 2017 Sep 12-14; Albena Resort, Bulgaria. Institute of Biology and Immunology of Reproduction, BAS, COST Action CA16119 and Mouseprint Ltd.; 2017. p. 64-5.