Centaurium erythraea extract improves redox-status and antioxidant enzyme activity of STZ-treated pancreatic beta cells and diabetic rat liver and kidney
2015
Аутори:
Đorđević, MilošDinić, Svetlana
Grdović, Nevena
Mihailović, Mirjana
Arambašić Jovanović, Jelena
Uskoković, Aleksandra
Tolić, Anja
Mišić, Danijela
Šiler, Branislav
Poznanović, Goran
Vidaković, Melita
Остала ауторства
Uzelac, BrankaТип документа:
Конференцијски прилог (Објављена верзија)
,
© 2015 by the Serbian Plant Physiology Society
Метаподаци
Приказ свих података о документуАпстракт:
Centaurium erythraea Rafn (CE) has long been used in traditional medicine in diabetes treatment. Since
oxidative stress plays a major role in development of diabetes and its complications, the main goal of This
study was to evaluate antioxidative properties of CE extract and its ameliorating effects in streptozotocin
(STZ)-induced diabetes. CE extract displayed strong hydrogen peroxide- and nitric oxide-scavenging activities,
as well as high reducing power in vitro. Treatment with CE extract improved redox-status of STZ-treated
rat pancreatic beta cells (Rin5F) by reducing DNA damage, lipid peroxidation and protein oxidation. This
was accompanied by repaired activity of antioxidative enzymes in STZ-treated Rin5F cells. CE-treatment lowered
STZ-induced increase in glutathione peroxidase (GPx) and manganese superoxide dismutase (MnSOD)
activities and partially restored decrease in catalase (CAT) activity. Improvement of redox-status was accompanied
by the increase of Rin5F cell viability and insulin expression. Administration of CE extract (100 mg kg-
1 orally, two weeks before and four weeks after diabetes induction) to STZ-induced diabetic rats exerted antioxidant
effects in liver and kidney. CE-treatment reduced DNA, lipid and protein damage and restored activity
of CAT, Mn/CuZnSOD and GPx that were disturbed in diabetic liver and kidney This improvement is reflected
by the preserved functional integrity of diabetic liver and kidney. CE-treatment reduced the level of
blood urea nitrogen and activity of liver transaminases (ALT and AST). According to these results, CE extract
has great potential in preventing diabetes and its complications, but additional experiments are needed in
order to reveal underlying mechanisms.
Кључне речи:
Cenraurium erythraea; diabetes melliitus; oxidative stress; antioxidant activityФинансирање / пројекти:
- Сигнални молекули у дијабетесу: идентификација потенцијалних биолошких маркера укључених у модификацију и интеграцију сигналних путева у циљу предикције и интервенције у дијабетесу (RS-MESTD-Basic Research (BR or ON)-173020)
У:
- Uzelac B, editor. Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia. Belgrade: Serbian Plant Physiology Society: Institute for Biological Research “Siniša Stanković”; 2015. p. 67.