Upregulation of glial markers with absence of a typical proinflammatory profile in the hippocampus of A53T mice as a model of Parkinson’s disease
2023
Preuzimanje 🢃
Autori:
Dubljević, OlgaPavković, Željko
Srbovan, Maja
Potrebić, Milica
Pešić, Vesna
Ostala autorstva
Kanazir, SelmaSavić, Danijela
Tip dokumenta:
Konferencijski prilog (Objavljena verzija)
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© 2023 by Serbian Neuroscience Society and associates
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Parkinson's disease (PD) is characterized by both motor and non-motor symptoms.
Current research indicates that neuroinflammation, along with activated glial cells,
represents one of the key factors in the pathogenesis of PD. The aim of this study was
to assess non-motor symptoms of PD as well as the expression of gene markers of
microgliosis, astrogliosis and neuroinflammation in a transgenic A53T mouse model,
which is recommended for studying PD and other synucleinopathies. These transgenic
mice express an A53T missense mutant form of human alpha-synuclein under the
control of the murine prion promoter.
Depression-like symptoms and anhedonia were assessed using Tail Suspension Test
and Sucrose Preference Test respectively, while memory deficits were assessed using
Novel Object Recognition Test along with Object Relocation Test. Gene expression of
ionized calcium-binding adapter molecule (Iba-1), glial fibrillary acidic protein
(GFAP α and δ form) and cytokines associated with the microglial pro-inflammatory
phenotype (TNFα, IL-1β, IL-6) was assessed using RT-PCR. Brain regions of interest
were the prefrontal cortex, hippocampus, and striatum of 6 months old A53T male
mice. Non-transgenic littermates were used as controls. Obtained results suggest that
A53T mice compared to non-transgenic littermates exert 1) the presence of memory
deficits and anhedonia, 2) no difference in the expression of glial activity markers and
pro-inflammatory cytokines in the cortex and striatum, and 3) increased expression of
glial activity markers in the hippocampus, but without significant changes in the
expression of cytokines. Overall, better understanding of the role of active glia in the
model is needed.
Ključne reči:
Neuroinflammation; Parkinson's disease; Microglia; AstrocytesFinansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200007 (Univerzitet u Beogradu, Institut za biološka istraživanja 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
U:
- Kanazir S, Savić D, editors. Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. Belgrade: Serbian Neuroscience Society; 2023. p. 109