MTORC1 signaling pathway changes under the effect of caloric restriction in the hippocampus of male Wistar rats
2023
Аутори:
Prvulović, MilicaTodorović, Smilja
Simeunović, Valentina
Vukojević, Anđela
Sokanović, Srđan
Jović, Milena
Milanović, Desanka
Mladenović, Aleksandra
Остала ауторства
Kanazir, SelmaSavić, Danijela
Тип документа:
Конференцијски прилог (Објављена верзија)
,
© 2023 by Serbian Neuroscience Society and associates
Метаподаци
Приказ свих података о документуАпстракт:
Caloric restriction (CR) is widely known for delaying age-related changes, but its
duration and onset can significantly alter its protective potential. One of the
underlying mechanisms of the effect of CR on aging is considered to be mTOR
pathway, an important player in nutrient sensing and regulation of cellular
metabolism.
Adult, middle-aged, and old male Wistar rats were exposed to CR (60% of AL) to
study the effect of CR of early (EOCR) and late onset (LOCR). Ad libitum (AL) fed
animals were used to test the effects of aging and as age-matched controls for the
effect of CR. Western blot was used to analyze the expression of proteins involved in
the signaling cascade of mTOR complex 1 (mTORC1) in rat hippocampus.
At 18 months of age, EOCR led to a decrease in phosphorylation of mTOR S2448,
whereas LOCR led to its increase at 24 months of age. pAkt T308 was decreased with
LOCR at 18 months, while at 24 months EOCR and LOCR increased its
phosphorylation. LOCR also led to an increased phosphorylation of p70S6K1 at 24
months of age.
Although EOCR led to a decrease in phosphorylation of mTOR at 18 months of age,
this effect was lost at 24 months, and was also not affecting the activation of the two
kinases targeting mTOR S2448. Negative effect of short-term LOCR at 24 months
was consistent in all examined proteins suggesting a caution when implementing CR
later in life.
Кључне речи:
Caloric restriction; Ageing; mTOR pathwayФинансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200017 (Универзитет у Београду, Институт за нуклеарне науке Винча, Београд-Винча) (RS-MESTD-inst-2020-200017)
У:
- Kanazir S, Savić D, editors. Book of abstracts: 8th Congress of Serbian Neuroscience Society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. Belgrade: Serbian Neuroscience Society; 2023. p. 74.