Short-term fish oil treatment increases number of microglial cells and expression level of TREM-2 in parietal cortex of 5XFAD mice
2023
Аутори:
Jović, MilenaSimeunović, Valentina
Vukojević, Anđela
Prvulović, Milica
Sokanović, Srđan
Mladenović, Aleksandra
Milanović, Desanka
Todorović, Smilja
Lončarević-Vasiljković, Nataša
Остала ауторства
Kanazir, SelmaSavić, Danijela
Тип документа:
Конференцијски прилог (Објављена верзија)
,
© 2023 by Serbian Neuroscience Society and associates
Метаподаци
Приказ свих података о документуАпстракт:
According to the amyloid hypothesis of Alcheimer’s disease (AD), disruption of
balance between production and clearance of Aβ from the cells leads to the
progressive accumulation and aggregation of amyloid beta (Aβ) peptides in the brain.
The glial system (microglial cells and astrocytes) is responsible for maintaining
homeostasis in the brain which implies its important role in the development and
progression of AD. Our previous work revealed that the short-term fish oil (FO)
treatment in 5xFAD mice, (AD animal model), reduces toxic Aβ load and increases
number of microglial/macrophage cells in parietal cortex. In the present study we
aimed to further decipher the roles of microglial and macrophage cells and to
elucidate possible mechanisms responsible for observed reduced level of toxic Aβ42
peptide. For this purpose, western blot and immunohistochemical analysis were used
to detect changes in parietal cortex of three-month-old 5xFAD mice after three weeks
FO treatment (100μl/animal/day). Distinction between microglial cells and
macrophages was assessed using double immunostaining with anti-TMEM119 and
anti-Iba1 antibodies respectively. Immunostaining was observed by confocal
microscopy. For western blot analysis anti-TREM-2 and anti-IDE were used to
observe potential mechanism responsible for extracellular clearance of toxic Aβ
forms. Quantification was done by Image Quant software. Our results showed that
short-term FO supplementation affects the localization and number of microglial cells
and macrophages. Macrophages were located around the plaque and were responsible
for the formation of a mechanical barrier, while microglial cells showed an increased
number under the treatment and were located far from the plaques. Furthermore, the
treatment did not seem to affect the level of IDE, while on the other hand it
significantly increased the level of TREM-2 (ultimately sustaining the microglial
response to Aβ-plaque-induced pathology).
Кључне речи:
Alzheimer's disease; Microglia; 5xFAD mice; Fish oilФинансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200017 (Универзитет у Београду, Институт за нуклеарне науке Винча, Београд-Винча) (RS-MESTD-inst-2020-200017)
У:
- Kanazir S, Savić D, editors. Book of abstracts: 8th Congress of Serbian Neuroscience Society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. Belgrade: Serbian Neuroscience Society; 2023. p. 65.