The effects of food restriction on anxiety level and dopaminergic system during aging in male Wistar rats
2019
Autori:
Prvulović, MilicaSmiljanić, Kosara
Todorović, Smilja
Kanazir, Selma
Mladenović, Aleksandra
Ostala autorstva
Saksida, TamaraStanisavljević, Suzana
Miljković, Đorđe
Tip dokumenta:
Konferencijski prilog (Objavljena verzija)
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© 2019 by the Institute for Biological Research "Siniša Stanković" – National Institute of Republic of Serbia, University of Belgrade
Metapodaci
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Aims: Food restriction (FR) ¬is well known as an environmental intervention efficient in delaying aging and age-related disorders. Important role in the regulation of food intake plays the gut-brain dopamine (DA) axis. Dopamine is a neurotransmitter involved in regulation of brain’s rewarding and pleasure centers, who’s signaling is indispensable to survival and maintenance of eating patterns. Reversely, reduced food intake affects DA circuits and behaviors controlled by DA, including anxiety. Herein we investigated mechanisms through which FR affects anxiety and the role of dopaminergic system in this process.
Methods: 60% FR of various onset and duration (FR1, FR2 and FR3) was implemented as a feeding regime for aging male Wistar rats. Open field test and light-dark box were used to investigate effects of age and food restriction on anxiety-like behavior. Western blot and PCR were used to determine the changes at the transcriptional and translational level.
Results: Open field test showed an increased general activity of animals under FR1 in comparison to the controls, while FR2 and FR3 seemed to have deleterious effect on anxiety level. Light-dark box confirmed deleterious effect of FR2 and FR3 regimens. Changes detected on behavioral level were accompanied with the specific changes in the level of dopaminergic receptors.
Conclusions: Our results showed that food restriction is not universally beneficial, but depends on age when implemented. We showed that FR-induced effects can vary from anxiolytic to anxiogenic, while the components of DA circuits in the brain show region-specific response to FR.
Ključne reči:
Food restriction; Dopamine; AgingU:
- Saksida T, Stanisavljević S, Miljković Đ, editors. Immunology at the Confluence of Multidisciplinary Approaches : abstract book: 2019 Dec 6-8; Belgrade, Serbia. Belgrade: Institute for Biological Research "Siniša Stanković", University of Belgrade; Immunological Society of Serbia; 2019. p. 73.