Somatostatin-14 influences pituitary–ovarian axis in peripubertal rats
2008
Autori:
Nestorović, NatašaManojlović-Stojanoski, Milica
Ristić, Nataša
Sekulić, Milka
Šošić-Jurjević, Branka
Filipović, Branko
Milošević, Verica
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
,
© 2008, Springer-Verlag
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
The effects of multiple somatostatin (SRIH-14) administration on the pituitary–ovarian axis were examined in peripubertal rats. Female Wistar rats received subcutaneously, two daily doses of 20 μg SRIH-14 per 100 g body weight (b.w.) for five consecutive days (from the 33rd to the 37th day of life). Follicle-stimulating (FSH), luteinizing (LH) and somatotropic (GH) cells were examined by the peroxidase–anti-peroxidase immunocytochemical method. Changes in cell volumes, volume densities and number per unit area (mm2) of FSH-, LH- and GH-immunoreactive cells were evaluated by stereology and morphometry. Serum FSH and LH levels were determined by RIA. Ovaries were analyzed by simple point counting of follicles. The volumes and volume densities of FSH-, LH- and GH-immunoreactive cells were significantly decreased while their numbers per mm2 remained unchanged. SRIH-14 induced a significant decrease in serum FSH and LH levels. In the ovary, SRIH-14 induced an increase in the number of primordial follicles, followed by a reduction in the number of small healthy growing follicles and absence of preovulatory follicles. The number of atretic follicles was unchanged. We concluded that treatment with SRIH-14 during the peripubertal period markedly inhibited pituitary FSH, LH and GH cells. In the ovary, SRIH-14 acted by inhibiting folliculogenesis without affecting atretic processes.
Ključne reči:
Somatostatin; Peripubertal rats; Gonadotropic cells; Somatotropic cells; OvaryIzvor:
Histochemistry and Cell Biology, 2008, 130, 699-708Finansiranje / projekti:
- Uticaj fitoestrogena, steroidnih i peptidnih hormona na ćelije neuroendokrinog sistema (RS-MESTD-MPN2006-2010-143007)
DOI: 10.1007/s00418-008-0442-7
ISSN: 0948-6143
PubMed: 18493786