Superoksid-dismutaze krompira (Solanum tuberosum L.): karakterizacija izoformi i ekspresija u uslovima visokih temperatura
Superoxide dismutases in potato (Solanum tuberosum L.): characterisation of isoforms and expression under high temperature
Abstract:
Potato (Solanum tuberosum L.) is one of the most important vegetable crops and is very sensitive to high temperatures. Heat stress causes an imbalance in the production and elimination of reactive oxygen species (ROS) in plant cells, leading to secondary oxidative stress. Superoxide dismutases (SODs) are important antioxidant enzymes involved in the defense of plant cells against ROS, which have not been studied in detail in potato so far. The results of this work revealed that the potato SOD gene family includes eight genes (StCuZnSOD1-3, StMnSOD and StFeSOD1-4), whose promoter regions and transcript exon-intron structure were examined in silico. In silico analysis also included physicochemical characterization of the StSOD proteins, identification of the SOD domains, assessment of the N-terminal signal in the peptides, prediction of the subcellular localization and tertiary structure of StSODs, and revealed the phylogenetic relationships between StSODs and SODs of other plant species. The expression of StSOD was investigated in commercial potato varieties grown in vitro during different temperature treatments. All eight StSOD genes were functional, and six genes were highly expressed. A temperature of 29 °C induced StSOD expression the most. Also, in ex vitro grown plants, an increased expression of StSOD was found under heat stress (30/20°C, day/night), mainly after the 7th day of treatment. It was noted that heat stress predominantly induces the expression of genes encoding chloroplast StSODs (StCuZnSOD2, StFeSOD2 and StFeSOD3) in leaves, genes encoding cytosolic (StCuZnSOD1) and mitochondrial (StMnSOD) StSODs in stolons, while it had no significant effect on the expression of StSODs in other organs. The obtained results indicate that StSOD plays an important role in the antioxidant protection of potato leaf and stolon cells under high temperature.
Keywords:
Solanum tuberosum L.; potato; superoxide dismutases; heat stress; oxidative stress; antioxydative enzymesSource:
Faculty of Biology, University of Belgrade, 2023, 1-114Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)