Polyphenol profile of honeydew and polyfloral honey from northern part of Montenegro
2019
Document Type:
Conference object (Published version)
,
© 2019 by the Faculty of Technology
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Show full item recordAbstract:
Introduction: Honey is a high energetic sweet food composed, among other components, of
bioactive compounds that attribute therapeutic properties of this product. As antioxidants,
polyphenols are recognized as the major constituents in honey responsible for its health
promoting properties. Bees produce a floral honey from the collected nectar of flowers, and
honeydew honey from collected excrement of insects or plants. Considering that Montenegro
is a country with traditional beekeeping, this is the first time to publish the results of its
honeydew and polyfloral honey samples.
Experimental: Analyzed honey samples, honeydew and polyfloral, were collected from three
locations in norther part of Montenegro, Berane, Bijelo Polje and Pljevlja. Polyphenol profile
of six honey samples was determinated by identifying polyphenols on UHPLC-LTQ OrbiTrap
MS, and by their quantifications on UHPLC-DAD MS/MS.
Results: Of the 32 identified polyphenol compounds, 24 were present in all samples. Appearing
the other eight polyphenol compounds has no constant diversity between the samples. The
similarity between them was confirmed by their quantifications. The highest proportion in total
polyphenols has a quercetin with a mean value of 3.82 mg/kg, followed by p-hydrohybenzoic
acid and p-coumaric acid. Slightly higher content of chrysin, pinocembrin, p-protocatechuic
and vanillic acid can be noticed. The quantifications of the target compounds include 33
polyphenols, and only 16 of them were previously identified. This indicates the occurrence of
certain dissimilarities between two used techniques, which are the consequences of similar
spectra of certain polyphenols and their derivatives.
Conclusion: This is the detailed study of polyphenols in Montenegrin honeys. Based on the
obtained results, it can be seen that the investigated honey samples are very rich in polyphenols,
with no significant differences between honeydew and polyfloral samples. Considering small
areas that cover these three regions of norther part of Montenegro, it can be assumed that small
diversity in plant species causes overlapping in honeybee’s pastures and similarity of samples
Keywords:
honey; honeydew; polyfloral honey; polyphenolsFunding / projects:
- Structure-properties relationships of natural and synthetic molecules and their metal complexes (RS-MESTD-Basic Research (BR or ON)-172017)
In:
- Vidović S, editor. Book of abstracts: 1st International Conference on Advanced Production and Processing: 2019 Oct 10-11; Novi Sad, Serbia. Novi Sad: Faculty of Technology; 2019. p. 39.