The effect of intermittent feeding on the number of parvalbumin-expressing neurons in the hippocampus of 5XFAD mice
2019
Аутори:
Srbovan, MajaPrpa, Ksenija
Tešić, Vesna
Milanović, Desanka
Perović, Milka
Kanazir, Selma
Остала ауторства
Isaković, AnđelkaТип документа:
Конференцијски прилог (Објављена верзија)
,
© 2019 by the Serbian Neuroscience Society
Метаподаци
Приказ свих података о документуАпстракт:
Aim: Food restriction has been widely associated with beneficial effects on brain aging and age-related neurodegenerative diseases such as Alzheimer’s disease (AD). In the present study, the effects of every-other-day (EOD) feeding regimen were studied in the hippocampus of 5XFAD mice, a well characterized animal model of AD. Parvalbumin (PV) inhibitory interneurons that are crucial for maintaining proper excitatory/inhibitory balance were examined.
Methods: Female 5xFAD mice (Tg) and their non-transgenic littermates (non-Tg) were exposed to ad libitum (AL) or intermittent, EOD feeding regimen, beginning at 2 months of age. Neurons expressing PV were detected by immunohistochemistry, in the dorsal hippocampus of 6-month-old animals. The number of parvalbumin-expressing neurons was determined independently in CA1, CA3, and DG hippocampal subregions.
Results: Immunohistochemical analysis revealed a substantial increase in the number of parvalbumin inhibitory neurons in the dorsal hippocampus of Tg-AL mice in comparison to non-Tg animals. In Tg-EOD mice, however, alterations in the number of PV-expressing neurons were subregion-specific comparing to Tg-AL mice of the same age.
Conlusions: The results of our study clearly indicate that PV-expressing interneurons are of importance in further understanding of neural basis of AD-like-associated cognitive impairments and EOD-induced effects in 5xFAD mouse model of AD.
Кључне речи:
Feeding; Parvalbumin-expressing neurons; Hippocampus; Alzheimer's diseaseФинансирање / пројекти:
- Пластичност мозга током старења: утицај дијеталне рестрикције и анестезије (RS-MESTD-Basic Research (BR or ON)-173056)
У:
- Isaković A, editor. Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia. Belgrade: Serbian Neuroscience Society; 2019. p. 299.