Antioxidant defence enzyme activities in some tissues of the freshwater bivalve Anodonta woodiana from the Sava river
2010
Authors:
Borković-Mitić, SlavicaPavlović, Slađan
Radovanović, Tijana
Gavrilović, Branka
Despotović, Svetlana
Gavrić, Jelena
Paunović, Momir
Pajović, Snežana
Saičić, Zorica
Contributors
Cvijić, GordanaAnđus, Pavle
Nedeljković, Nadežda
Radenović, Lidija
Đorđević, Jelena
Korać, Bato
Document Type:
Conference object (Published version)
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© 2010 University of Belgrade, Faculty of Biology
Metadata
Show full item recordAbstract:
In the present study we have used the activity of aantioxidative defence enzymes: superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and the activity of glutathione S-transferase (GST), an enzyme of the phase II biotransformation process to evaluate their utility as biological toll for pollution monitoring in the Sava River. We also determined total protein content, as well as protein and SOD electrophoretic profiles in the digestive gland and the gills of the freshwater bivalve anodonta woodiana. Obtained results indicate a significant influence of tissue specifity of the all investigated enzyme activities. The activities of SOD, CAT, GSH-Px and phase II biotransformation enzyme GST were significantly higher in the digestive gland in respect to the gills, whereas GR activity exhibited higher levels in the gills. This was most likely a reflection of different metabolic activities and different responses to environmental conditions of the examined tissues. Our study also suggest, that the variation of SOD expression pattern in Anodonta woodiana could be used as a tool for the environmental monitoring. Our wo bivalve Anodonta woodiana friom the Sava Riverrk represents a first study of this kind and showed that the antioxidant defence enzymes can be considered as a potential biomarkers of the freshwater
Keywords:
Oxidative stress; Gills; Digestive gland; Anodonta woodiana; Sava RiverFunding / projects:
- Proučavanje poremećaja homeostaze i određivanje biomarkera oksidacionog stresa kod aerobnih organizama (RS-MESTD-MPN2006-2010-143035)
In:
- Cvijić G, Anđus P, Nedeljković N, Radenović L, Đorđević J, Korać B, editors. Book of Abstracts: International Symposium One hunderd years of Ivan Djajać's (Jean Diaja) Belgrade School of Physiology; 2010 Sep 10-14; Belgrade, Serbia. Belgrade: University of Belgrade, Faculty of Biology; 2010. p. 95.