Effects of acute Cd-treatment on antioxidant defense system in the blood of rats: protective role of vitamin E
2003
Autori:
Ognjanović, BrankaPavlović, Slađan
Maletić, Snežana
Žikić, Radoslav V.
Štajn, Andraš Š.
Saičić, Zorica
Ostala autorstva
Đurić, DraganTip dokumenta:
Konferencijski prilog (Objavljena verzija)
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© 2003 by the Serbian Physiological Society
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Cadmium (Cd) is an ubiquitous toxic metal that is involved in a variety of pathological condicions. The present study investigated the po-,sible protective role of vitamin E (Vit E) in cadmium induced oxidative stress in the blood of rats. Male, Wistar albino rats 3 months old (weighing 280 ± 30 g) were used in our experiments. Rats were injected with a single dose (1) of CdClz (0,4 mg Cd/kg b.m., i.p., 24h before the sacrificing), (2)Vit E + Cd (20 UI Vit E/kg b.m., i.m., 48h +0,4 mg Cd/kg b.m., i.p., 24h before the sacrificing). The group of animals without treatment was control. The activities of enzymatic components of AOS: copper zinc containing superoxide-dismutase (CuZn SOD), catalase (CAT), glutathione-peroxidase (GSH
Px), glutathione-reductase (GR) and glutathione-S-transferase (GST) were analyzed
in erythrocytes and concentrations of non-enzymatic components of AOS: reduced '
glutathione (GSH), ascorbic acid (AsA) and vitamin E (Vit E), as well as lipid peroxide (LP) concentration were determined in the blood of rats. Acute intoxication I of rats with Cd were followed by significantly increased LP and increased activities I
of enzymatic, as well as concentrations of non-enzymatic components of AOS in blood. The pretreatment with vitamin E after acute intoxication of rats with Cd partialy improved components of AOS. Our results indicate that liposoluble antioxidant (Vit E) resulted as suitable and potent antioxidant action, probably as consequence of quenchening of reactive oxygen species, inhibiton of lipid peroxidation and prevention of free-radical mediated injuries. The results of this study suggested that vitamin E functions as antioxygenic nutrients.
Ključne reči:
Cadmium; Antioxidant defense enzymes; Vit E; Blood; RatsFinansiranje / projekti:
- Serbian Ministry of Sciences, Yugoslavia, Grants No. 03E23 and 03E18.
U:
- Đurić D, editor. Abstract Book: Conference of Physiological Sciences with international participation: Risk Factors and Health: from Molecule to the Scientific Basis of Prevention; 2003 Nov 7-9; Belgrade, Zrenjanin, Serbia. Belgrade: Serbian Physiology Society; 2003. p. 69.