Hippocampal Antioxidative System in Epilepsy
2019
Аутори:
Ristić, AleksandarSavić, Danijela
Bogdanović Pristov, Jelena
Brkljačić, Jelena
Baščarević, Vladimir
Raičević, Savo
Savić, Slobodan
Остала ауторства
Isaković, AnđelkaТип документа:
Конференцијски прилог (Објављена верзија)
,
© 2019 by the Serbian Neuroscience Society
Метаподаци
Приказ свих података о документуАпстракт:
Mesial temporal lobe epilepsy associated with hippocampal sclerosis (mTLE-HS) is probably the single most frequent human focal epilepsy. The involvement of redox processes in the pathological mechanisms of mTLE-HS has been implicated by mitochondrial dysfunction and oxidative damage, and by different metabolic abnormalities that have been observed in sclerotic hippocampi, such as altered metabolism of redox-active metals. The strongest proof came with the analysis of enzymatic antioxidative system. Sclerotic hippocampi show drastically increased activity and levels of hydrogen peroxide–removing enzymes – catalase and glutathione peroxidase/reductase. Catalase is located mainly in neurons in both, controls and HS. Sclerotic hippocampi are depleted of glutathione peroxidase-positive blood vessels that are present in control hippocampi. Pertinent to this, it has been documented that hippocampi of mTLE-HS patients show increased blood vessel density, but most of the vessels represent atrophic vascular structures. On the other hand, HS showes specific glutathione peroxidase-rich loci that are present in gyrus dentatus, CA regions, and alveus, and appear to represent bundles of astrocytes. These loci are probably sites of excessive (neuronal) production of hydrogen peroxide that is counteracted by astrocytes. Finally, protein levels of mitochondrial enzyme manganese superoxide dismutase are higher in HS than controls. Neurons with abnormal morphology and strong superoxide dismutase immunofluorescence are present in all neuronal layers in HS. In close, antioxidative system is upregulated in HS implying that epileptogenic hippocampi are exposed to oxidative stress. The involvement of redox alterations in the pathology of epilepsy may open new pharmacologic perspectives for mTLE-HS treatment.
Финансирање / пројекти:
- Ћелијска и молекулска основа неуроинфламације: потенцијала циљна места за транслациону медицину и терапију (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41014)
- Молекуларни механизми редокс сигналинга у хомеостази, адаптацији и патологији (RS-MESTD-Basic Research (BR or ON)-173014)
У:
- Isaković A, editor. Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia. Belgrade: Serbian Neuroscience Society; 2019. p. 42.