Interleukin-17 stimulates inducible nitric oxide synthase activation in rodent astrocytes
2001
Аутори:
Trajković, VladimirStošić-Grujičić, Stanislava
Samardzić, Tatjana
Marković, Miloš
Miljković, Đorđe
Ramić, Zorica
Mostarica Stojković, Marija
Тип документа:
Чланак у часопису (Објављена верзија)
,
© 2001 Elsevier Science B.V.
Метаподаци
Приказ свих података о документуАпстракт:
The effect of interleukin-17 (IL-17) on production of nitric oxide (NO) in rodent astrocytes was investigated. While IL-17 by itself did not induce NO production, it caused a dose-dependent enhancement of IFN-gamma-triggered NO synthesis in both mouse and rat primary astrocytes. In contrast, IL-17 was unable to stimulate NO synthesis in either murine or rat macrophages. IFN-gamma-triggered expression of mRNA for iNOS, but not for its transcription factor interferon regulatory factor-1 (IRF-1), was markedly elevated in IL-17-treated astrocytes. The induction of iNOS mRNA by IL-17 in IFN-gamma-pretreated astrocytes was abolished by antagonists of nuclear factor-kappaB (NF-kappaB) activation--a proteasome inhibitor MG132 and an antioxidant agent PDTC, as well as with specific p38 MAP kinase inhibitor SB203580. While IL-17 stimulated both IL-1beta and IL-6 production in astrocytes, only IL-1 was partly responsible for IL-17-induced NO release. Finally, IL-17 synergized with exogenous IL-1beta and TNF-alpha for astrocyte NO production. Having in mind a well-known neurotoxic action of NO, these results suggest a possible role for IL-17 in the inflammatory diseases of the CNS.
Кључне речи:
IL-17; Astrocyte; Nitric oxide; iNOSИзвор:
Journal of Neuroimmunology, 2001, 119, 2, 183-191Финансирање / пројекти:
- Ministry of Science and Technology, Republic of Serbia, Yugoslavia
DOI: 10.1016/s0165-5728(01)00391-5
ISSN: 0165-5728
PubMed: 11585620