Biofilm Adhesion and Micromorphology Analysis after Professional Oral Hygiene Procedures on CAD/CAM Milled and Veneered Zirconia Restorations: In Vitro Study
2023
Autori:
Perić, MirjanaČairović, Aleksandra
Špadijer Gostović, Aleksandra
Stanimirović, Dragan
Kostić, Marina
Ivanov, Marija
Ćirić, Ana
Glamočlija, Jasmina
Pavlović, Vladimir B.
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
The purpose of this study was to evaluate the surface texture and biofilm adhe sion of veneered or CAD/CAM milled zirconia (partially stabilized with yttrium) after professional
oral hygiene procedures. The samples (4 × 4 mm, thickness 2 mm; n = 72) were separated from
zirconia blanks (3Y-TZP-LA). One group was veenered with ceramics, and the other group of samples
was CAD/CAM milled. Each group had two subgroups: polished and glazed. The samples were
subjected to simulated strokes of professional brushing using abrasive paste and ultrasonic scaling.
The parameters of surface micromorphology and receptivity to biofilm were calculated before and
after simulating the given methods of the professional maintenance of oral hygiene. Scanning electron
microscopy (SEM) was used to evaluate zirconia surface properties. Microbial (bacterial/fungal)
species (Staphylococcus aureus, Streptococcus sanguinis and Candida albicans) were used and cultured
on respective sterilized zirconia surfaces. Colony-forming unit (CFU) counts were used to quantify
the amount of biofilm formation on zirconia samples surfaces. Results: The SEM analysis showed
the greatest change in surface microtopography after the use of ultrasonic scaling on glazed zirconia
samples. Less formation of colonies on the surfaces of CAD/CAM milled zirconia restorations was
observed. Conclusion: Routine methods of oral hygiene professional maintenance can damage the
surfaces of glazed zirconia restorations.
Ključne reči:
zirconia; CAD/CAM; ultrasonic scaling; SEM; surface microtopography; microbial biofilmIzvor:
Applied Sciences, 2023, 13, 14, 8389-Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200007 (Univerzitet u Beogradu, Institut za biološka istraživanja 'Siniša Stanković') (RS-MESTD-inst-2020-200007)