Приказ основних података о документу
5-Aza-2'-deoxycytidine stimulates inducible nitric oxide synthase induction in C6 astrocytoma cells
dc.creator | Stojanović, Ivana D. | |
dc.creator | Popadić, Dušan | |
dc.creator | Vučković, Olivera | |
dc.creator | Harhaji-Trajković, Ljubica | |
dc.creator | Miljković, Đorđe | |
dc.creator | Trajković, Vladimir | |
dc.date.accessioned | 2023-07-31T10:41:49Z | |
dc.date.available | 2900-01-01 | |
dc.date.issued | 2004 | |
dc.identifier.issn | 0006-8993 | |
dc.identifier.uri | http://radar.ibiss.bg.ac.rs/handle/123456789/6009 | |
dc.description.abstract | The influence of a nucleoside analog 5-aza-2'-deoxycytidine (5-AzadC) on inducible nitric oxide synthase (iNOS)-dependent nitric oxide (NO) production in various rat cell types was investigated. In C6 astrocytoma cell line and primary astrocytes, 5-AzadC enhanced proinflammatory cytokine (IFN-gamma, TNF-alpha, IL-1)-triggered NO synthesis in a time- and dose-dependent manner. In contrast, 5-AzadC did not potentiate NO production in IFN-gamma-stimulated macrophages, fibroblasts, or endothelial cells. Blockade of transcription or translation in C6 cells abolished the observed effect, suggesting the iNOS gene expression, rather than its catalytic activity, as a target for the drug action. Accordingly, 5-AzadC upregulated IFN-gamma-induced expression of iNOS mRNA in C6 astrocytes. The effect of 5-AzadC on astrocyte NO release was blocked by the inhibitor of p44/42 mitogen activated protein kinase-dependent signaling. Finally, the observed stimulatory effect of 5-AzadC on iNOS expression was apparently independent of DNA demethylation, as DNA digestion with methylation-sensitive restriction enzyme HpaII showed that 5-AzadC failed to demethylate cellular DNA in conditions used for iNOS induction. | sr |
dc.language.iso | en | sr |
dc.publisher | Elsevier B.V. | sr |
dc.relation | The Ministry of Science, Technology and Development of The Republic of Serbia (Grants No. 1664 and No. 2020) | sr |
dc.rights | restrictedAccess | sr |
dc.source | Brain Research | sr |
dc.subject | 5-Aza-2V -deoxycytidine | sr |
dc.subject | Astrocyte | sr |
dc.subject | Nitric oxide | sr |
dc.subject | iNOS | sr |
dc.title | 5-Aza-2'-deoxycytidine stimulates inducible nitric oxide synthase induction in C6 astrocytoma cells | sr |
dc.type | article | sr |
dc.rights.license | ARR | sr |
dc.rights.holder | © 2003 Elsevier B.V. | sr |
dc.citation.issue | 1 | |
dc.citation.volume | 998 | |
dc.identifier.doi | 10.1016/j.brainres.2003.11.014 | |
dc.identifier.pmid | 14725971 | |
dc.identifier.scopus | 2-s2.0-0346023052 | |
dc.identifier.wos | 000188796500010 | |
dc.citation.spage | 83 | |
dc.citation.epage | 90 | |
dc.type.version | publishedVersion | sr |
dc.citation.rank | M22 |