The effects of cadmium and coenzyme Q10 on superoxide dismutase, catalase and glutathione peroxidase activities in red blood cells of rats
2000
Аутори:
Pavlović, SlađanOgnjanović, Branka
Štajn, Andraš Š.
Žikić, Radoslav V.
Saičić, Zorica
Petrović, Vojislav M.
Тип документа:
Конференцијски прилог (Објављена верзија)
,
© 2000 by the Polish Biophysical Society, Łódź Branch
Метаподаци
Приказ свих података о документуАпстракт:
The effects of chronic exposure to cadmium (Cd) on the superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activities and the protective role of coenzyme Q10 (CoQ10) in red blood cells (RBC) of two months old male Wistar albino rats were examined. The animals were exposed to Cd (200 mg CdCl2 x H2O, in drinking water and 100 μL olive oil, i.m., every fifth day), CoQ10 (20 mg/kg CoQ10 dissolved in olive oil, i.m., every fifth day), Cd+ CoQ10 (200 mg CdCl2 x H2O, in drinking water + 20 mg/kg CoQ10 dissolved in olive oil, i.m., every fifth day) and olive oil (100 μL olive oil, i.m., every fifth day) in course of 30 days. The average intake of Cd was 17 mg/day/kg body mass and CoQ10 16 mg/kg/dose. After the treatment the animals were sacrificed without the anesthaesia and blood was collected and prepared for enzyme analysis.
The activity of SOD was significantly decreased in animals treated with olive oil when compared to the controls. At the other hand, in comparison to the animals treated with olive oil, the SOD activity was significantly increased in rats treated with Cd, CoQ10, and Cd+CoQ10. CAT activity was significantly decreased in rats exposed to Cd+CoQ10 and olive oil in respect to the control animals, while in comparison to the animals treated with olive oil CAT activity was significantly increased in animals treated with Cd, CoQ10 and Cd+CoQ10. In comparison to the controls the GSH-Px activity was significantly decreased in RBC of rats exposed to CoQ10 and olive oil. In respect to the animals treated with olive oil, the activity of GSH-Px was increased in animals treated with Cd, CoQ10 and Cd+CoQ10.
Our results show that cadmium administered with olive oil did not exhibit toxic effects on SOD, CAT and GSH-Px activities in RBC of rats. At the same time, CoQ10 by quenching of free oxygen radicals and by inhibition of lipid peroxidation may improve the antioxidant defense enzyme activities in RBC of rats. Our investigations also show that olive oil exhibit some protective effects on SOD, CAT and GSH-Px activities in red blood cells of rats.
Кључне речи:
Cadmium; Coenzyme Q10; Superoxide dismutase; Catalase; Glutathione peroxidase; RatsФинансирање / пројекти:
- Serbian Ministry of Sciences, Yugoslavia, Grants No. 03E23 and 03E18.
У:
- Abstracts: 5th Symposium Free Radicals in Biology and Medicine; 2000 Jun 7-10; Łódź, Poland. Łódź: Polish Biophysical Society, Łódź Branch; 2000. p. 206.