Testosterone effects on the pituitary gonadotropic cells in an animal model of the andropause
2018
Authors:
Ajdžanović, VladimirJakovljević, Vladimir
Miler, Marko
Ristić, Nataša
Potrebić, Milica
Sretenović, Jasmina
Popovski Perčinić, Florina
Pendoski, Laze
Milošević, Verica
Contributors
Jakovljević, VladimirĐurić, Dragan
Živković, Vladimir
Document Type:
Conference object (Published version)
,
© 2018 by Serbian Physiological Society
Metadata
Show full item recordAbstract:
The aim of this study was to investigate the immunohistomorphometric parameters of follicle-stimulating hormone (TSH) and luteinizing hormone (I.H) cells after testosterone application in an animal model of the andropause. Middle-aged Wistar rats were divided into orchidectomized (Or) and testosterone treated orchidectomized (OrxT) groups. Testosterone propionate (5 mg/kg b.m. (day) was administered for three weeks. while Or group received the vehicle alone.
Immunohistochemically stained FSH and I cells were subjected to the morphometric and optical density-related analysis. while circulating concentration of testosterone was measured by the immunoassay. Serum concentration of testosterone was increased (ps0.05) by 24 fold in comparison with Orx group.
Testosterone treatment did not cause significant changes of body mass or absolute and relative pituitary weights compared to Or group. The volume of FSH and LH cells was decreased (ps0.05) by 51.3% and 56.6% respectively, in comparison with Or rats. Relative volume density of FSI and LE cells was also decreased (p<0.05) by 54.0% and 72.8% respectively, compared to Or group. Results related to the optical density of gonadotropic cells were in line with the morphometric findings i.e. this parameter of FSH and LH cells was decreased (P«0.05) by 25.7% and 16.2% respectively, in comparison with Or rats. In conclusion. applied testosterone decreased morphometric parameters and optical density of gonadotropic cells in Or rats.
Funding / projects:
- The effects of select plant extracts, phytoestrogens, steroid and peptide hormones on the rat neuroendocrine system (RS-MESTD-Basic Research (BR or ON)-173009)
In:
- Đurić DM, Jakovljević V, Živković V, editors. Abstract book: 4th Congress of Physiological Sciences of Serbia with International Participation: Current Trends in Physiological Sciences: from cell signals to the biology of aging; 2018 Sep 19-23; Niš, Serbia. Belgrade: Serbian Physiological Society; 2018. p. 118.