Immunohistomorphometric characteristics of pituitary GH cells in infant and peripubertal female rats after treatment with estradiol of human chorionic gonadotropin
2015
Preuzimanje 🢃
Autori:
Ristić, NatašaAjdžanović, Vladimir
Trifunović, Svetlana
Tanić, Nasta
Bujišić, Nada
Milošević, Verica
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Th e eff ects of estradiol-dipropionate (EDP) or human chorionic gonadotropin (hCG) on immunohistomorphometric characteristics of pituitary GH cells in infant and peripubertal female rats were investigated. Th e fi rst group of females received fi ve injections of EDP (0.25 mg/kg b.w.) during the neonatal period of life, and was further divided into two subgroups which were sacrifi ced at the infantile period (17th day) or at the peripubertal period (38th day). Th e second group received two doses of hCG (50 IU/kg b.w.) on the 15th and 16th day of life in the fi rst subgroup, and on the 36th and 37th days of life in the second subgroup, while they were sacrifi ced 24 h after the last treatment, respectively. Th e control females were injected with an equivalent volume of the vehicle and sacrifi ced according to the appropriate schedules as the hormone treated rats. EDP treatment decreased GH cell volume density in infant and peripubertal females, by 38% and 76% (p<0.05) respectively, in comparison with the controls. Th e number of GH cells per mm2 in infantile and peripubertal period was decreased in EDP treated animals by 26% and 53% (p<0.05) respectively, compared to the controls. Also, upon EDP treatment in both periods, GH cells were diminished in size and less intensely immunolabelled than in the control groups. Th e morphometric parameters in animals treated with hCG were insignifi cantly changed in both analyzed periods, in comparison with the controls. Unlike hCG, EDP manifested clear inhibitory eff ects on the immunohistomorphometric characteristics of GH cells in examined female rats.
Ključne reči:
female rat; infant; peripubertal; GH cells; estradiol,; human chorionic gonadotropinIzvor:
Serbian Journal of Experimental and Clinical Research, 2015, 16, 4, 281-285Finansiranje / projekti:
- Odgovor neuroendokrinog sistema pacova na odabrane biljne ekstrakte, fitoestrogene, steroidne i peptidne hormone (RS-MESTD-Basic Research (BR or ON)-173009)