Anti-inflammatory effects of benfotiamine in LPS stimulated microglia: role of NF-κB signaling
2015
Аутори:
Božić, IvaSavić, Danijela
Laketa, Danijela
Nedeljković, Nadežda
Peković, Sanja
Lavrnja, Irena
Остала ауторства
Lukić, MiodragJonjic, Stipan
Nikolich-Zugich, Janko
Тип документа:
Конференцијски прилог (Објављена верзија)
,
© 2015 by the Immunological Society of Serbia
Метаподаци
Приказ свих података о документуАпстракт:
Microglial cells are immune cells of the central nervous system (CNS) that
play a major role in its surveillance. Changes in CNS homeostasis, invading
pathogens or neuron impairment, lead to activation of microglial cells that
quickly proliferate, acquire amoeboid morphology and produce toxic
mediators such as nitric oxide (NO) and pro-inflammatory cytokines. These
changes are regulated by transcription factors, most importantly NF-κB.
Although microglial activation is important for maintaining tissue
homeostasis, excessive activation leads to chronic inflammation that can
damage healthy neurons. Substances that suppress microglial activation are
potential therapeutics for neurodegenerative diseases. Benfotiamine (Sbenzoylthiamine-
O-monophosphate) is a synthetic derivative of vitamin B1
that has anti-inflammatory properties. In this study we investigated antiinflammatory
properties of benfotiamine on activated microglia in vitro.
BV-2 microglia were pre-treated with benfotiamine, stimulated with LPS
and their viability and morphology were evaluated. LPS induced prominent
alterations in cell morphology, enlargement of cell bodies and spreading of
multiple processes. Pre-treatment with benfotiamine before LPS
stimulation suppressed these morphological changes. Additionally,
benfotiamine diminished LPS induced NO production, without altering cell
viability. Furthermore, benfotiamine decreased LPS induced production of
pro-inflammatory cytokines TNF- α and IL-6, while increasing production
of anti-inflammatory cytokine IL-10. Analysis of NF-κB activation
revealed that benfotiamine's effects were mediated by this transcription
factor. In conclusion, benfotiamine exerts anti-inflammatory properties in LPS
activated microglia in vitro and should be further investigated as a potential
therapeutic for neurodegenerative diseases.
Финансирање / пројекти:
- Ћелијска и молекулска основа неуроинфламације: потенцијала циљна места за транслациону медицину и терапију (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41014)
У:
- Lukić M, Jonjic S, Nikolich-Zugich J, editors. 3rd Belgrade EFIS symposium on Immunoregulation: Immunity, Infection, Autoimmunity and Aging; 2015 May 24-27; Arandjelovac, Serbia. Belgrade: Immunological Society of Serbia; 2015. p. 35.