α2-macroglobulin expression during the acute-phase response in malnourished rats
Authors:
Uskoković, AleksandraMihailović, Mirjana
Dinić, Svetlana
Grdović, Nevena
Vidaković, Melita
Arambašić Jovanović, Jelena
Bogojević, Desanka
Ivanović Matić, Svetlana
Martinović, Vesna
Petrović, Miodrag
Poznanović, Goran
Grigorov, Ilijana
Contributors
Đurić, DraganJakovljević, Vladimir
Document Type:
Conference object (Published version)
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© 2007 by the Serbian Physiological Society
Metadata
Show full item recordAbstract:
During the acute-phase (AP) response, the synthesis rate and serum concentration of
the AP protein a 2-macroglobulin (MG) increases in the rat. MG gene transcription is
mainly controlled by the inflammatory cytokine IL-6 and related trans- factors, the
Signal Transducers and Activators of Transcription (STAT) and CCAAT/Enhancer
Binding Protein (C/EBP) . Nearly every aspect of the body's defense system· is changed in malnutrition (MN). We examined whether MN affected rat MG synthesis
and STAT3 and C/EBP expression. MN was induced in male Wistar rats .by 50%
food restriction during six weeks. The AP response was induced by turpentine oil injection (1 µl/g body weight). Using "rocket" immuno electrophoresis we observed that, compared to the well-nourished (WN) control, MN was associated wit increased serum MG. The AP response promoted a further rise in se rum MG. The STAT3 and C/EBP levels in the nuclear extract were examined by immunoblote analysis. In rats with MN, STAT3 was elevated and during the AP response, increased STAT3 levels were observed in both WN and MN rats. The C/EBP levels were similar in MN and WN rats however the AP response induced its significants, increase in rats with MN. Obtained results suggested that malnutrition contribute to an increased MG expression by influencing IL-6 signal transduction pathway.
Malnutrition-related increased of MG synthesis could be connected with positive regulatory role of STAT3 , while the additional AP-related MG increased synthesis in malnourished rats require activit y of C/EBP .
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Funding / projects:
- Akutni i hronični stres: mehanizmi regulacije homeostaze u akutnoj radijacionoj bolesti i dijabetesu (RS-MESTD-MPN2006-2010-143002)
In:
- Đurić D, Jakovljević V, editors. Abstract book / a Scientific Conference with International Participation "Nutrition, Treatment and Cardiovascular Risk Management"; 2007 May 24-27; Novi Sad, Serbia. Belgrade: Serbian Physiological Society; 2007. p. 111.