Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression
2016
Аутори:
Prodanović, RadišaKorićanac, Goran
Vujanac, Ivan
Đorđević, Ana
Pantelić, Marija
Romić, Snježana
Stanimirović, Zoran
Kirovski, Danijela
Тип документа:
Чланак у часопису (Нерецензирана верзија)
,
© 2016 by the authors
Метаподаци
Приказ свих података о документуАпстракт:
We investigated the hypothesis that obesity in dairy cows enhanced expression of proteins involved in hepaticfatty acid uptake and metabolism. Sixteen Holstein-Friesian close-up cows were divided into 2 equal groupsbased on their body condition score (BCS) as optimal (3.25 ≤ BCS ≤ 3.5) and high (4.0 ≤ BCS ≤ 4.25). Intravenousglucose tolerance test (GTT) and liver biopsieswere carried out at day 10 before calving. Blood samples were collectedbefore (basal) and after glucose infusion, and glucose, insulin and non-esterified fatty acid (NEFA) levelswere determined at each sample point. In addition, β-hydroxybutyrate and triglycerides levels were measuredin the basal samples. The liver biopsieswere analyzed for total lipid content and protein expression of insulin receptorbeta (IRβ), fatty acid translocase (FAT/CD36) and sterol regulatory element-binding protein-1 (SREBP-1).Basal glucose and insulin were higher in high-BCS cows, which coincided with higher circulating triglyceridesand hepatic lipid content. Clearance rate and AUC for NEFA during GTT were higher in optimal-BCS cows. The developmentof insulin resistance and fatty liver in obese cows was paralleled by increased hepatic expression ofthe IRβ, CD36 and SREBP-1. These results suggest that increased expression of hepatic CD36 and SREBP-1 is relevantin the obesity-driven lipid accumulation in the liver of dairy cows during late gestation.
Напомена:
This is a submitted version of the following article: Prodanović R, Korićanac G, Vujanac I, Djordjević A, Pantelić M, Romić S, Stanimirović Z, Kirovski D. Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression. Res Vet Sci. 2016;107:16–9. http://dx.doi.org/10.1016/j.rvsc.2016.04.007.
Кључне речи:
dairy cows; fatty liver; CD36; Sterol regulatory element-binding protein-1Извор:
Research in Veterinary Science, 2016, 107, 16-19Финансирање / пројекти:
- Молекуларно-генетичка и екофизиолошка истраживања у заштити аутохтоних анималних генетичких ресурса, очувању добробити, здравља и репродукције гајених животиња и производњи безбедне хране (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46002)
- Улога стероидних хормона у неуроендокриној адаптацији на стрес и патофизиологији метаболичког синдрома - молекуларни механизми и клиничке импликације (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41009)
Повезане информације:
- Друга верзија
https://radar.ibiss.bg.ac.rs/handle/123456789/6085
DOI: 10.1016/j.rvsc.2016.04.007
ISSN: 0034-5288
PubMed: 27473969