High Fat Diet Exaggerate Metabolic and Reproductive PCOS Features by Promoting Oxidative Stress: An Improved EV Model in Rats
2023
Аутори:
Rakić, DejanaJoksimović Jović, Jovana
Jakovljević, Vladimir
Živković, Vladimir
Nikolić, Maja
Sretenović, Jasmina
Nikolić, Marina
Jović, Nikola
Bićanin Ilić, Marija
Arsenijević, Petar
Dimitrijević, Aleksandra
Vulović, Tatjana
Ristić, Nataša
Bulatović, Kristina
Bolevich, Sergej
Stijak, Lazar
Pantović, Suzana
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Polycystic ovary syndrome (PCOS) is a frequent multifactorial
endocrinopathy affecting women in the reproductive period, often associated with infertility and
metabolic disorders. The use of animal models helps to better understand etiopathogenesis, enabling
the examination of the effects of certain drugs in order to discover the best possible therapeutic
approach. We tried to investigate the additional effect of estradiol-valerate (EV) and high-fat diet
(HFD) in female rats to explore PCOS-related alterations with special focus on oxidative stress.
Materials and Methods: Animals were divided into three groups: control group (CTRL, n = 6), estradiolvalerate
group (EV, n = 6), and estradiol-valerate group on HFD (EV + HFD, n = 6). PCOS was
induced by single subcutaneous injection of long-acting EV in a dose of 4 mg/per rat. We tried to
improve the metabolic characteristics of the PCOS animal model by adding HFD, so the CTRL and
EV group had a regular diet, while the EV + HFD group had HFD during the induction period of
60 days. Results: We observed alterations of anthropometric parameters and hormonal disturbances,
along with estrus cycle impairment reassembly to obese-type PCOS phenotype. Moreover, glucose
metabolism was impaired after addition of HFD to EV protocol, contrary to EV administered alone.
Histological analysis confirmed more numerous cystic follicles after the combination of EV and HFD
protocol. The alterations of oxidative stress markers could be related to and serve as the mechanistic
base for development of PCOS-related endocrine, reproductive, and metabolic properties. Conclusions:
The additive effect of EV and HFD was obvious in the majority of the parameters observed. Our
study strongly demonstrated metabolic as well as reproductive properties of PCOS in rats.
Кључне речи:
ovary; PCOS; oxidative stress; estradiol-valerate; high-fat dietИзвор:
Medicina, 2023, 59, 6, 1104-Финансирање / пројекти:
- Junior Project Faculty of Medical Sciences, University of Kragujevac, Serbia, Junior Project number 12/22.
DOI: 10.3390/medicina59061104
ISSN: 1010-660X
PubMed: 37374308