Dietary restriction affectsS ACTH Cells of peripubertal female rats
2010
Аутори:
Milošević, VericaAjdžanović, Vladimir
Trifunović, Svetlana
Bogojević, Desanka
Ivanović-Matić, Svetlana
Martinović, Vesna
Grigorov, Ilijana
Тип документа:
Конференцијски прилог (Објављена верзија)
,
© 2010 by the The Society of Medical Biochemists of Serbia
Метаподаци
Приказ свих података о документуАпстракт:
Dietary restriction (DR) in female rats pre sumably activates the stress (hypothalamo-pituitary adrenal) system. It's well known that various pituitary cell types are involved in the reaction to stress, i.e. adrenocorticotropic hormone (ACTH), prolactin and growth hormone cells. The aim of this study was to examine the effects of DR on the immunohistomor phometric features of pituitary ACTH cells and blood levels of ACTH. Peripubertal (38 days old) female Wistar rats were housed one per cage, under standard environmental conditions (12 h light/dark cycle, 22 ±
2 °C) with food and water ad libitum. ·Experimental
females were fed with 50% of the total amount of food consumed by the controls, during 6 weeks. The ACTH cells were stained using the peroxidase-antiperoxidase immunohistochemical procedure and stereological analyses were conducted. The blood ACTH was measured by radioimmunoassay (ACTH-IMMULITE kit). In the experimental group body weight was (p<0.05) decreased by 48.8%, while the relative pituitary weight was (p<0.05) increased by 76 .9% in comparison with corresponding parameters ir1 controls . The volume of ACTH cells, as well as their volume density was significantly increased (p< 0.05 ) in the experimental group, by 17 .6% and 1 2.5% respectively. The blood ACTH level was increased (p<0.05) in experimental group by 13.4% . Our findings show that the DR in peripubertal female rats stimulates the stress system, which was confirmed by increased morphofunctional parameters of ACTH ce lls .
.
Финансирање / пројекти:
- Акутни и хронични стрес: механизми регулације хомеостазе у акутној радијационој болести и дијабетесу (RS-MESTD-MPN2006-2010-143002)
У:
- 17th Congress of Medical Biochemistry and Laboratory Medicine, with international participations, 6th EFCC Symposium for Balkan Region; 2010 Oct 5-9; Belgrade, Serbia. Belgrade: Society of Medical Biochemists of Serbia. Belgrade: Society of Medical Biochemists of Serbia; 2010. p. 454-5. (Journal of Medical Biochemistry; Vol. 29; No. 4).