Alterations of antioxidant defense system in some tissues of rats treated with selenium
2001
Аутори:
Ognjanović, BrankaŽikić, Radoslav V.
Štajn, Andraš Š.
Pavlović, Slađan
Saičić, Zorica
Petrović, Vojislav M.
Тип документа:
Конференцијски прилог (Објављена верзија)
,
© 2001 by the Institute of Biophysics, Chinese Academy of Sciences
Метаподаци
Приказ свих података о документуАпстракт:
We investigated the activity of antioxidant enzymes: superoxide dismutases - total (Tot SOD), copper zinc containing (CuZn SOD) and manganese containing (Mn SOD), catalase (CAT), glutathione peroxidase (GSH-PX) and glutathione-S-transferase (GST) in the liver, kidneys, heart and interscapular brown adipose tissue (IBAT), as well as contents of ascorbic acid (AsA) and vitamin E (Vit E) in the liver and kidneys of male two months old Wistar albino rats. The animals were divided in two experimental groups: control animals (C) and animals treated with selenium (Se). The rats treated with selenium were drank water _c ontaining 0.1 µg/mL selenium (Se) as Na-selenite during 30 days. The average daily intake of 7 µg Se/kg body mass was calculated from the water consumed. Both experimental groups consisted of seven animals. The obtained results were compared in respect to the control animals. .
Our results show that Tot SOD and CuZn SOD activities were significantly increased in the liver (p<0.02), kidneys (p<0.005), heart (p<0.005) and IBAT (p<0.05) of Se treated animals, while the activity of Mn SOD was significantly increased only in the liver (p<0.02). CAT-activitywas statistically increased in the liver (p<0.02) of animals treated with Se, while the GSH-Px activity was significantly increased only in the heart (p<0.02). The activity of GST was significantly increased in the liver (p<0.02), kidneys (p<0.02) and heart (p<0.005) of Se treated animals.
The obtained results show that AsA content in the liver and kidneys was significantly increased (p<0.02 and p<0.05, respectively). The content of Vit E was also significantly increased in the liver (p<0.005) and kidneys (p<0.005) of animals exposed to Se.
Presented results show that oral intake of Se as Na-selenite, improves antioxidant defense system by increasing antioxidant enzyme activities, as well as, by elevation of AsA and Vit E contents in the all examined tissues of rats.
Кључне речи:
Antioxidant defense system; Rats; SeleniumФинансирање / пројекти:
- Serbian Ministry of Sciences, Yugoslavia, Grants No. 03E23 and 03E18.
У:
- Programme and Abstracts: The Second International Symposium on Natural Antioxidants: Molecular Mechanisms and Health Effects; 2001 Jun 4-8; Beijing, China. Institute of Biophysics, Chinese Academy of Sciences; 2001. p. 169.