Somatic embryo regeneration from filaments of Aesculus flava Sol.
2012
Authors:
Zdravković-Korać, SnežanaMilojević, Jelena
Tubić, Ljiljana
Devrnja, Nina
Ćalić, Dušica
Contributors
Rakonjac, LjubinkoDocument Type:
Conference object (Published version)
,
© 2012 by the Institute of Forestry, Belgrade, Serbia
Metadata
Show full item recordAbstract:
Somatic embryogenesis is a powerful technique widely used for propagation of elite genotypes, especially mature, difficult-to-propagate woody species, like ornamental species of the genus Aesculus. For the purpose of clonal propagation of A. flava, callus was induced from the stamen filaments cultured in darkness on MS medium with variable levels of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-furfurylaminopurine (kinetin, Kin). Among the 2,4-D/Kin combinations tested, 5 μM 2,4 D + 5 μM Kin was optimal combination for the highest callus growth. Some of the friable filament-derived calli were dispersed in liquid MS medium supplemented with 5 μM 2,4 D + 5 μM Kin, to induce cell suspensions. After 8 weeks of culture, the callusing filaments were transferred to solid plant growth regulator (PGR) - free medium, while microcalli in suspensions were spun down, resuspended in semisolid PGR-free medium and dispensed in Petri-dishes. Both types of cultures were exposed to 16h light photoperiod. Somatic embryogenesis was achieved by the two techniques after approximately 4 weeks, but cell suspension was a more efficient method. Both 2,4-D and Kin were indispensable for the induction of callus competent for somatic embryogenesis. Primary somatic embryos obtained by the two methods exhibited a great potential of secondary somatic embryogenesis, yielding numerous secondary somatic embryos. Cyclic somatic embryogenesis allowed the establishment of permanent embryogenic cultures.
Keywords:
Filaments; Cell suspension; Somatic embryogenesis; Tissue culture; Yellow buckeyeFunding / projects:
- Biotechnology in vitro - crop, medicinal and endangered plant species (RS-MESTD-Basic Research (BR or ON)-173015)
In:
- Rakonjac Lj, editor. Proceedings: International Scientific Conference: Forest in the Future - Sustainable Use, Risks and Challenges; 2012 Oct 4-5; Belgrade, Serbia. Belgrade: Institute of Forestry; 2012. p. 379-87.