Chemical composition, antioxidant and antigenotoxic activities of Cotinus coggygria stem extract
2013
Autori:
Matić, SanjaStanić, Snežana
Bogojević, Desanka
Solujić, Slavica
Mladenović, Milan
Stanković, Nevena
Mihailović, Vladimir
Katanić, Jelena
Mihailović, Mirjana
Ostala autorstva
Vinterhalter, DraganTip dokumenta:
Konferencijski prilog (Objavljena verzija)
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© 2013 by the Serbian Plant Physiology Society
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
The plant Cotinus coggygria Scop. (family Anacardiaceae) is commonly used in folk medicine for the treatment of various illnesses, such as diarrhea, paradontosis, gastric and duodenal ulcers. Different parts of this plant have been subjected to pharmacological evaluation for their potential antihaemorragic, wound-healing, antiinflammatory, and antimicrobial activity. The present study was undertaken to investigate the phenolic conten according to the Folin-Ciocalteu procedure, while the spectrophotometric method with aluminium chloride was used for the determination of total ftavonoids. The phenolic and flavonoid composition of extract were determined by the HPLC method. Antioxidant activiy was quantitatively determined using a DPPH radical scavenging assay. To examine the antigenotoxic potential using the comet assay, Wistar rats were treated with 100 mg/ kg body weight of pyrogallol, which possesses a potent ability to generate free radicals and induce oxidative stress. The content of total phenols and ftavonoids was 3.78 mg of gallic acid/g of dry plant material and 8.29 mg of rutin/g of dry plant material, respectively. HPLC analysis showed that myricetin was the dominant compound (511.5 µg/g), while hydroxyl derivatives of cinnammic acids (chlorogenic, caffeic, coumaric, ferulic and rosmaric acid) were identified in the extract in varying amount. The neutralisation of DPPH radicals was up to 95%, while the maximum inhibition concentration is approximately 125 µg/ml. The dose of 500 mg/kg body weight of the extract, that display no genotoxic activity, and its main flavonoid compound myricetin, in equivalent amount as present in the extract, were applied intraperitoneally either 2 or 12 h prior to pyrogallol. As measured by the decrease in total score and tail moment, the DNA damage in liver was reduced by the extract and myricetin.
Finansiranje / projekti:
- Simultana bioremedijacija i soilifikacija degradiranih prostora, za očuvanje prirodnih resursa biološki aktivnih supstanci i razvoj i proizvodnju biomaterijala i dijetetskih proizvoda (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-43004)
- Signalni molekuli u dijabetesu: identifikacija potencijalnih bioloških markera uključenih u modifikaciju i integraciju signalnih puteva u cilju predikcije i intervencije u dijabetesu (RS-MESTD-Basic Research (BR or ON)-173020)
- Određivanje atomskih parametara na osnovu oblika spektralnih linija (RS-MESTD-MPN2006-2010-141010)
U:
- Vinterhalter D, editor. Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia. Belgrade: Serbian Plant Physiology Society; 2013. p. 90-1.