Phytochemical profiling by UHPLC-DAD/±HESI-MS/MS analyzes and hepatoprotective activity of Gentiana cruciata L. against CCl4 induced liver injury in Wistar rats
2013
Preuzimanje 🢃
Autori:
Mihailović, VladimirMišić, Danijela
Katanić, Jelena
Mihailović, Mirjana
Solujić, Slavica
Stanković, Vesna
Mladenović, Milan
Stanković, Nevena
Ostala autorstva
Sovilj, SofijaDekanski, Aleksandar
Tip dokumenta:
Konferencijski prilog (Objavljena verzija)
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© 2013 by the Serbian Chemical Society
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Many Gentiono species are known for their pharmaceutical values, such as Gentiono crucioto L, commonly called cross gentian [1]. The dried roots and above-ground parts of G. crucioto are consumed in the Balkan region as herbal tea or a medicinal wine for loss of appetite, as a stomachic and component in preparations showing beneficial effects in gall and liver diseases [2]. This study using in vivo model investigates hepatoprotective activity of G. crucioto aerial part methanol extract (GCA) against carbon tetrachloride-induced liver injury in rats. Wistar rats were orally pretreated with GCA (100, 200, and 400 mg/kg) and silymarin (100 mg/kg) for seven days before they were treated with CCl4 (1 ml/kg, 1:1 mixture in olive oil) which caused liver injury. Separation, determination and quantification of components in GCA was performed using Dionex Ultimate 3000 UHPLC system equipped with a diode array detector (DAD) and connected to a triple-quadrupole mass spectrometer. Pretreatment with GCA dose-dependently and significantly (p < 0.001) decreased levels of serum transaminases, alkaline phosphatase and total bilirubin, whereas an increase was found in the level of total protein compared with CCl4-treated group. In the liver tissue antioxidant studies, we found a significant increase in the levels of catalase, superoxide dismutase and reduced glutathione, whereas there was marked reduction in the levels of thiobarbituric acid-reactive substances, as compared to CCl4 treated group. Histological analyses also show that GCA reduced the incidence of liver lesions including necrosis, ballooning degeneration and micro- and macro-vesicular changes induced by CCl4 in rats. GCA was characterized by the presence of sweroside, swertiamarin, gentiopicrin, loganic acid, isovitexin 4',7-diglucoside, orientin and vitexin, as revealed by UHPLC-DAD-MS and UHPLC-MS/MS analyses. Quantification of targeted compounds in the SRM (selected reaction monitoring) experiment of UHPLC-MS/MS analysis clearly indicated that gentiopicrin (1.067%) was the dominant secoiridoid glycoside in GCA, whereas concentrations of sweroside (0.064%) and swertiamarin (0.033%) were significantly lower.
Finansiranje / projekti:
- Simultana bioremedijacija i soilifikacija degradiranih prostora, za očuvanje prirodnih resursa biološki aktivnih supstanci i razvoj i proizvodnju biomaterijala i dijetetskih proizvoda (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-43004)
- Fiziološka, hemijska i molekularna analiza diverziteta odabranih retkih i ugroženih biljnih vrsta u cilju ex situ zaštite i produkcije biološki aktivnih jedinjenja (RS-MESTD-Basic Research (BR or ON)-173024)
U:
- Sovilj S, Dekanski A, editors. Book of abstracts: 8th International Conference of the Chemical Societies of the South-East European Countries: ICOSECS 8; 2013 Jun 27-29; Belgrade, Serbia. Belgrade: Serbian Chemical Society; 2013. p. 220.