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Phytochemical profiling by UHPLC-DAD/±HESI-MS/MS analyzes and hepatoprotective activity of Gentiana cruciata L. against CCl4 induced liver injury in Wistar rats
dc.contributor | Sovilj, Sofija | |
dc.contributor | Dekanski, Aleksandar | |
dc.creator | Mihailović, Vladimir | |
dc.creator | Mišić, Danijela | |
dc.creator | Katanić, Jelena | |
dc.creator | Mihailović, Mirjana | |
dc.creator | Solujić, Slavica | |
dc.creator | Stanković, Vesna | |
dc.creator | Mladenović, Milan | |
dc.creator | Stanković, Nevena | |
dc.date.accessioned | 2023-10-02T14:36:10Z | |
dc.date.available | 2023-10-02T14:36:10Z | |
dc.date.issued | 2013 | |
dc.identifier.isbn | 978-86-7132-053-5 | |
dc.identifier.uri | http://radar.ibiss.bg.ac.rs/handle/123456789/6142 | |
dc.description.abstract | Many Gentiono species are known for their pharmaceutical values, such as Gentiono crucioto L, commonly called cross gentian [1]. The dried roots and above-ground parts of G. crucioto are consumed in the Balkan region as herbal tea or a medicinal wine for loss of appetite, as a stomachic and component in preparations showing beneficial effects in gall and liver diseases [2]. This study using in vivo model investigates hepatoprotective activity of G. crucioto aerial part methanol extract (GCA) against carbon tetrachloride-induced liver injury in rats. Wistar rats were orally pretreated with GCA (100, 200, and 400 mg/kg) and silymarin (100 mg/kg) for seven days before they were treated with CCl4 (1 ml/kg, 1:1 mixture in olive oil) which caused liver injury. Separation, determination and quantification of components in GCA was performed using Dionex Ultimate 3000 UHPLC system equipped with a diode array detector (DAD) and connected to a triple-quadrupole mass spectrometer. Pretreatment with GCA dose-dependently and significantly (p < 0.001) decreased levels of serum transaminases, alkaline phosphatase and total bilirubin, whereas an increase was found in the level of total protein compared with CCl4-treated group. In the liver tissue antioxidant studies, we found a significant increase in the levels of catalase, superoxide dismutase and reduced glutathione, whereas there was marked reduction in the levels of thiobarbituric acid-reactive substances, as compared to CCl4 treated group. Histological analyses also show that GCA reduced the incidence of liver lesions including necrosis, ballooning degeneration and micro- and macro-vesicular changes induced by CCl4 in rats. GCA was characterized by the presence of sweroside, swertiamarin, gentiopicrin, loganic acid, isovitexin 4',7-diglucoside, orientin and vitexin, as revealed by UHPLC-DAD-MS and UHPLC-MS/MS analyses. Quantification of targeted compounds in the SRM (selected reaction monitoring) experiment of UHPLC-MS/MS analysis clearly indicated that gentiopicrin (1.067%) was the dominant secoiridoid glycoside in GCA, whereas concentrations of sweroside (0.064%) and swertiamarin (0.033%) were significantly lower. | sr |
dc.language.iso | en | sr |
dc.publisher | Belgrade: Serbian Chemical Society | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/43004/RS// | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173024/RS// | sr |
dc.rights | openAccess | sr |
dc.source | Book of abstracts: 8th International Conference of the Chemical Societies of the South-East European Countries: ICOSECS 8; 2013 Jun 27-29; Belgrade, Serbia | sr |
dc.title | Phytochemical profiling by UHPLC-DAD/±HESI-MS/MS analyzes and hepatoprotective activity of Gentiana cruciata L. against CCl4 induced liver injury in Wistar rats | sr |
dc.type | conferenceObject | sr |
dc.rights.license | ARR | sr |
dc.rights.holder | © 2013 by the Serbian Chemical Society | sr |
dc.description.other | Sovilj S, Dekanski A, editors. Book of abstracts: 8th International Conference of the Chemical Societies of the South-East European Countries: ICOSECS 8; 2013 Jun 27-29; Belgrade, Serbia. Belgrade: Serbian Chemical Society; 2013. p. 220. | sr |
dc.citation.spage | 220 | |
dc.type.version | publishedVersion | sr |
dc.identifier.cobiss | 43496207 | |
dc.identifier.fulltext | https://radar.ibiss.bg.ac.rs/bitstream/id/15084/bitstream_15084.pdf | |
dc.citation.rank | M34 | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_ibiss_6142 |