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Regulation of rat haptoglobin gene expression is coordinated by the nuclear matrix
dc.creator | Vidaković, Melita | |
dc.creator | Dinić, Svetlana | |
dc.creator | Grdović, Nevena | |
dc.creator | Mihailović, Mirjana | |
dc.creator | Uskoković, Aleksandra | |
dc.creator | Quesada, Piera | |
dc.creator | Poznanović, Goran | |
dc.date.accessioned | 2023-10-03T08:51:01Z | |
dc.date.available | 2900-01-01 | |
dc.date.issued | 2009 | |
dc.identifier.issn | 0730-2312 | |
dc.identifier.uri | http://radar.ibiss.bg.ac.rs/handle/123456789/6147 | |
dc.description.abstract | Using computer stress-induced duplex destabilization (SIDD) analysis and binding experiments, we identified a S/MAR element (−599/−200 bp) (Hp-S/MAR) adjacent to the cis-element (−165/−56 bp) in the rat haptoglobin gene. We examined its functional interactions with the lamins and lamin-associated proteins in the basal state and during acute-phase (AP) response-induced increased transcription. Colocalization, electrophoretic mobility shift assay (EMSA), and re-electrophoresis of nucleoprotein complexes, South-Western and Western blot analysis and coimunoprecipitation experiments revealed that the lamins, PARP-1, C/EBPβ, and Hp-S/MAR assembled higher order complexes through direct lamin-Hp-S/MAR and probably PARP-1–Hp-S/MAR interactions although C/EBPβ did not bind to the Hp-S/MAR but established direct interaction with PARP-1. The transition from constitutive to increased haptoglobin gene transcription during the AP response was associated with quantitative and qualitative changes in Hp-S/MAR–protein interactions, respectively, observed as increased association of the lamin(s) with the Hp-S/MAR and as the appearance of a 90 kDa Hp-S/MAR-binding protein. Also, during the AP response the contact between C/EBPβ and PARP-1 established in the basal state was lost. DNA chromatography with the haptoglobin cis-element and Western blot analysis suggests that PARP-1 was a coactivator during constitutive and elevated transcription. The results show that the lamin components of the nuclear matrix form a network of functional, dynamic protein–protein and protein–Hp-S/MAR associations with multiple partners, and underline the involvement of PARP-1 in the regulation of haptoglobin gene transcription. We concluded that the interplay of these interactions fine tunes haptoglobin gene expression to meet the changing requirements of liver cells. | |
dc.publisher | Wiley | en |
dc.rights | restrictedAccess | |
dc.source | Journal of Cellular Biochemistry | en |
dc.subject | Acute phase | |
dc.subject | C/EBPβ | |
dc.subject | Haptoglobin | |
dc.subject | Lamins | |
dc.subject | Nuclear matrix | |
dc.subject | PARP-1 | |
dc.subject | S/MAR | |
dc.title | Regulation of rat haptoglobin gene expression is coordinated by the nuclear matrix | en |
dc.type | article | en |
dc.rights.license | ARR | |
dc.rights.holder | © 2009 Wiley‐Liss, Inc. | |
dc.citation.issue | 6 | |
dc.citation.volume | 107 | |
dc.identifier.doi | 10.1002/jcb.22225 | |
dc.identifier.pmid | 19521970 | |
dc.identifier.scopus | 2-s2.0-68049113342 | |
dc.identifier.wos | 000268826900018 | |
dc.citation.spage | 1205 | |
dc.citation.epage | 1221 | |
dc.type.version | publishedVersion | |
dc.citation.rank | M22 |