A comparison between anther culture and microspore suspension culture of Aesculus flava
2010
Authors:
Zdravković-Korać, SnežanaĆalić, Dušica
Milojević, Jelena
Tubić, Ljiljana
Vinterhalter, Branka
Contributors
Rakonjac, LjubinkoDocument Type:
Conference object (Published version)
,
© 2010 by the Institute of Forestry, Belgrade, Serbia
Metadata
Show full item recordAbstract:
Anthers of A. flava were isolated from disinfected flower buds and cultivated on solid callus induction medium (CIM), containing Murashige and Skoog's (MS) mineral solution with 1 mg/l 2,4 dichlorophenoxyacetic acid (2,4 D) and 1 mg/l 6 furfurylaminopurine (Kin). A half of anthers was kept on solid CIM medium and the other half was macerated 3-5 days later, to create microspore suspension cultures. The microspores released from anthers were suspended in liquid CIM medium, sieved through a 50 μm mesh and shaken on a platform shaker. Eight weeks later, anthers were transferred to regeneration induction medium (RIM) containing MS salts, 0.01 mg/l 2,4 D and 1 mg/l Kin. Microspore suspensions were mixed with equal volumes of cooled RIM and the mixture was dispensed in Petri dishes. Embryo emergence from both types of cultures had been monitored over a 2-month period. The difference in embryogenic potential of anthers positioned along the inflorescence's axis was observed and it declined acropetally. Anthers isolated from basal third of inflorescence (A-anthers) exhibited the highest frequency of embryo formation (36.6%), comparing to anthers from the middle third of inflorescence (B-anthers) and particularly the top third anthers (C-anthers). The embryo average number per anther did not differ significantly between A- and B-anthers, whereas it was significantly lower in C-anthers. The same tendency was observed in microspore suspension cultures. Microspore suspension culture was more efficient method for embryo induction, as it yielded twice more embryos then anther culture. Androgenic embryos obtained by both techniques were maintained and multiplied by repetitive somatic embryogenesis on solid RIM medium.
Keywords:
androgenesis; anther culture; microspore suspension; yellow buckeyeFunding / projects:
- Regulacija morfogenetskih procesa i sekundarnog metabolizma i genetičke transformacije biljaka u kulturi in vitro (RS-MESTD-MPN2006-2010-143026)
In:
- Rakonjac Lj, editor. Proceedings: International Scientific Conference: Forest Ecosystems and Climate Changes: Volume 2; 2010 Mar 9-10; Belgrade, Serbia. Belgrade: Institute of Forestry; 2010. p. 49-54.