Paraquat-mediated oxidative stress in Nepeta pannonica L.
2015
Autori:
Cvetković, JelenaMilutinović, Milica
Božunović, Jelena
Aničić, Neda
Nestorović Živković, Jasmina
Živković, Suzana
Mišić, Danijela
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
The toxicity of bipyridynium herbicide paraquat (PQ) is related to its rapid generation of superoxide in a chain reaction and the production of reactive oxygen species (ROS) in chloroplasts under light conditions. The nature and magnitude of the cellular responses to oxidative stress effects of five paraquat concentrations (0.05-20 μM) on in vitro grown Nepeta panonnica shoots were investigated. The results revealed that relative abundances of Mn SOD and Cu/Zn SOD isoforms changed dose-dependently, whereas catalase (CAT) and peroxidase (POX) activities increased along with paraquat concentrations. Polyphenol oxidase (PPO) activity was progressively inhibited, and, consequently, phenolic compounds were accumulated. Rosmarinic acid (RA) and an unidentified caffeic acid derivative (UCAD) were found to be the most abundant phenolics in shoots of this species. Paraquat was shown to be a potent elicitor that can stimulate accumulation of potent bioactive compounds in Nepeta pannonica, such is rosmarinic acid.