Differential regulation of glutamine synthetase and glutamate synthase genes by plant growth regulators in Arabidopsis
2013
Authors:
Dragićević, MilanBogdanović, Milica
Milutinović, Milica
Filipović, Biljana
Todorović, Slađana
Simonović, Ana
Contributors
Vinterhalter, DraganDocument Type:
Conference object (Published version)
,
© 2013 by the Serbian Plant Physiology Society
Metadata
Show full item recordAbstract:
The glutamine synthetase (GS) - glutamate synthase (GOGAT) cycle is responsible for primary and secondary ammonium assimilation in plants. In the Arabidopsis genome there are five genes encoding cytoplasmic GS1 isoforms (GLN1;1-1;5), one gene encoding chloroplastic GS2 isoform (GLN2), two genes encoding ferredoxin-dependent GOGAT isoforms (GLU1 and GLU2), and one gene encoding NADH dependent GOGAT isoform (GLT1). These genes are distinctly regulated during plant development and respond differently to environmental cues to ensure plasticity in the plants responses to changes in nitrogen availability, light and metabolic state of the cell. In this work we investigated the expression of GS and GOGAT genes in Arabidopsis plants treated with different concentrations of plant growth regulators: kinetin, abscisic acid, gibberellic acid and 2,4-dichlorophenoxyacetic acid. Four week old plants grown in liquid culture were treated for 24h with these growth regulators and the expression of GS and GOGAT genes in roots and shoots was measured by qPCR with absolute quantification. The results show that plant growth regulators differentially regulate the expression of GS and GOGAT genes. In many instances, the observed patterns of GS and GOGAT gene expression in plants treated with different growth regulators provides insights into the hormonal regulation of these genes during development and as response to changes in nitrogen and light availability.
Keywords:
glutamine synthetase; Arabidopsis; glutamate synthaseFunding / projects:
- Physiological, chemical and molecular analysis of the diversity of selected rare and endangered plant species and application of biotechnology for ex situ conservation and production of biologically active compounds (RS-MESTD-Basic Research (BR or ON)-173024)
In:
- Vinterhalter D, editor. Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Belgrade. Belgrade: Serbian Plant Physiology Society; 2013. p. 41.